Rabbit anti-Human, Mouse FMR1 Polyclonal Antibody | anti-FMR1 antibody

FMR1, NT (Fragile X Mental Retardation 1 Protein, Protein FMR-1, FMRP) (FITC)

Cat. Num. AAA6476822

• Gene Names: FMR1; POF; FMRP; POF1; FRAXA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FMR1; Polyclonal Antibody; NT (Fragile X Mental Retardation 1 Protein; Protein FMR-1; FMRP) (FITC); anti-FMR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FMR1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FMR1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 20-48 in the N-terminal region of human FMR1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse lung tissue lysates (35ug/lane) usingMBS625326. FMR1 (arrow) was detected usingMBS625326.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS625326, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625326 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS625326 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FMR1 antibody

Antibodies; RNA Binding Proteins

References

1. Yuhas, J., et.al., Genet Test Mol Biomarkers 13(6), 855-859 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503765
• NCBI GeneID: 2332
• NCBI Accession #: NP_002015.1
• NCBI GenBank Nucleotide #: NM_002024.5
• UniProt Accession #: Q06787; Q16578; Q5PQZ6; Q99054; A6NNH4; D3DWT0; D3DWT1; D3DWT2; G8JL90
• NCBI Official Full Name: fragile X mental retardation protein 1 isoform ISO1
• NCBI Official Synonym Full Names: fragile X mental retardation 1
• NCBI Official Symbol: FMR1
• NCBI Official Synonym Symbols: POF; FMRP; POF1; FRAXA
• NCBI Protein Information: fragile X mental retardation protein 1
• UniProt Protein Name: Fragile X mental retardation protein 1
• Protein Family: Mat- sexual cell fertilization-promoting factor
• UniProt Gene Name: FMR1
• UniProt Synonym Gene Names: FMRP; Protein FMR-1
• UniProt Entry Name: FMR1_HUMAN

NCBI Description

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]