Western Blot (WB)
(WB: Bafl-3 cell lysate was probed with MBS616026 at 1ug/ml)
Rabbit anti-Mouse, Human Flt3 Polyclonal Antibody | anti-Flt3 antibody
Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135) (MaxLight 650)
Gene Names
Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
Reactivity
Mouse, Human
Applications
Immunoprecipitation, Western Blot, Immunocytochemistry
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Flt3; Polyclonal Antibody; Cytoplasmic (Fms-like Tyrosine Kinase 3; CD135) (MaxLight 650); anti-Flt3 antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the processed (130kD) and the mature forms (155kD) of mouse Flt3. Species Crossreactivity: human
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-Flt3 antibody
Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
IP: 4ug immunoprecipitated Flt3 from 500ug of mouse Bafl-3 RIPA lysate.
Applications are based on unconjugated antibody.
ICC: IgG, H&L (FITC) Pab Gt xRb
WB: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Applications are based on unconjugated antibody.
ICC: IgG, H&L (FITC) Pab Gt xRb
WB: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunogen
His-tag fusion protein corresponding to aa786-1000 of mouse Flt3.
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: Bafl-3 cell lysate was probed with MBS616026 at 1ug/ml)
Western Blot (WB)
(WB: Bafl-3 cell lysate was probed with MBS616026 at 1ug/ml)
Product Categories/Family for anti-Flt3 antibody
References
1. Beslu, N., et al., J. Biol. Chem. 271: 20075-20081, 1996. 2. Maroc, N., et al., Oncogene 8: 909-918, 1993.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112,640 Da
NCBI Official Full Name
receptor-type tyrosine-protein kinase FLT3
NCBI Official Synonym Full Names
FMS-like tyrosine kinase 3
NCBI Official Symbol
Flt3
NCBI Official Synonym Symbols
Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
NCBI Protein Information
receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; fetal liver kinase 2; tyrosine-protein kinase receptor flk-2
UniProt Protein Name
Receptor-type tyrosine-protein kinase FLT3
Protein Family
UniProt Gene Name
Flt3
UniProt Synonym Gene Names
Flk-2; Flt-3; FLK-2; FLT-3
UniProt Entry Name
FLT3_MOUSE
Uniprot Description
Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.5 PublicationsManual assertion based on experiment in:Ref.5
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationManual assertion based on experiment in:Ref.10
Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. FLT3LG binding leads to dimerization and activation by autophosphorylation.1 PublicationManual assertion based on experiment in:Ref.10
Research Articles on Flt3
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Product Notes
The Flt3 flt3 (Catalog #AAA6476681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135) (MaxLight 650) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Flt3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC). IP: 4ug immunoprecipitated Flt3 from 500ug of mouse Bafl-3 RIPA lysate. Applications are based on unconjugated antibody. ICC: IgG, H&L (FITC) Pab Gt xRb WB: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the Flt3 flt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flt3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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