Rabbit anti-Human FLT3 Polyclonal Antibody | anti-FLT3 antibody

FLT3, NT (Tyrosine-protein Kinase Receptor FLT3, Fms-like Tyrosine Kinase 3, FLT-3, STK1, FL Cytokine Receptor, Stem Cell Tyrosine Kinase 1, STK-1, CD135) (Biotin)

Cat. Num. AAA6476642

• Gene Names: FLT3; FLK2; STK1; CD135; FLK-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: FLT3; Polyclonal Antibody; NT (Tyrosine-protein Kinase Receptor FLT3; Fms-like Tyrosine Kinase 3; FLT-3; STK1; FL Cytokine Receptor; Stem Cell Tyrosine Kinase 1; STK-1; CD135) (Biotin); anti-FLT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FLT3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FLT3 antibody: Flow Cytometry (FC/FACS), FLISA
• Application Notes: FC/FACS: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 33-64 in the N-terminal region of human FLT3, UniProt Accession #P36888.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis using F54600-07A in 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western Blot analysis of MBS6006370 in HL-60 cell lysate. FLT3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS6006370, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Product Categories/Family for anti-FLT3 antibody

Antibodies; Tyrosine Kinases

References

1. Minami, Y., et al., Blood 102(8):2969-2975 (2003). 2. Zwaan, C.M., et al., Blood 102(7):2387-2394 (2003). 3. Sitnicka, E., et al., Blood 102(3):881-886 (2003). 4. Galimberti, S., et al., Anticancer Res. 23(4):3419-3426 (2003). 5. Kottaridis, P.D., et al., Leuk. Lymphoma 44(6):905-913 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 121114304
• NCBI GeneID: 2322
• NCBI Accession #: NP_004110.2
• NCBI GenBank Nucleotide #: NM_004119.2
• UniProt Accession #: P36888; Q13414; A0AVG9; B7ZLT7; B7ZLT8; F5H0A0
• Molecular Weight: 112,903 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 3
• NCBI Official Symbol: FLT3
• NCBI Official Synonym Symbols: FLK2; STK1; CD135; FLK-2
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Flt3 receptor-interacting lectin
• UniProt Gene Name: FLT3
• UniProt Synonym Gene Names: CD135; FLK2; STK1; FLK-2; FLT-3; STK-1
• UniProt Entry Name: FLT3_HUMAN

NCBI Description

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jul 2008]

Uniprot Description

FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum lumen; cytosol; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; vascular endothelial growth factor receptor activity; protein binding; protein homodimerization activity; protein complex binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; glucocorticoid receptor binding

Biological Process: negative regulation of B cell differentiation; lymphocyte proliferation; pro-B cell differentiation; organ regeneration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of phosphoinositide 3-kinase activity; response to organic nitrogen; positive regulation of phosphoinositide 3-kinase cascade; regulation of apoptosis; negative regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; myeloid progenitor cell differentiation; positive regulation of MAPKKK cascade; B cell differentiation; positive regulation of cell proliferation; hemopoiesis; pro-T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; leukocyte homeostasis

Disease: Leukemia, Acute Lymphoblastic