Rabbit anti-Human FLCN Polyclonal Antibody | anti-FLCN antibody

FLCN, CT (Folliculin, Birt-Hogg-Dube Syndrome Protein, BHD Skin Lesion Fibrofolliculoma Protein, BHD) (PE)

Cat. Num. AAA6476741

• Gene Names: FLCN; BHD; FLCL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FLCN; Polyclonal Antibody; CT (Folliculin; Birt-Hogg-Dube Syndrome Protein; BHD Skin Lesion Fibrofolliculoma Protein; BHD) (PE); anti-FLCN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FLCN.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FLCN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human FLCN (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis MBS623543 pre-incubated without (lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. FLCN (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(F5730-01 immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-FLCN antibody

Antibodies; Disease Markers

References

1. Khoo, S.K., et.al., J. Med. Genet. 39(12), 906-912 (2002). 2. Shin, J.H., et.al., J. Med. Genet. 40(5), 364-367 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22907034
• NCBI GeneID: 201163
• NCBI Accession #: NP_659434.2
• NCBI GenBank Nucleotide #: NM_144997.5
• UniProt Accession #: Q8NFG4; Q6ZRX1; Q96BD2; Q96BE4; A6NJJ8
• Molecular Weight: 64,473 Da
• NCBI Official Full Name: folliculin isoform 1
• NCBI Official Synonym Full Names: folliculin
• NCBI Official Symbol: FLCN
• NCBI Official Synonym Symbols: BHD; FLCL
• NCBI Protein Information: folliculin; birt-Hogg-Dube syndrome protein; BHD skin lesion fibrofolliculoma protein
• UniProt Protein Name: Folliculin
• Protein Family: Folliculin
• UniProt Gene Name: FLCN
• UniProt Synonym Gene Names: BHD

NCBI Description

This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.