Rabbit FLCN Polyclonal Antibody | anti-FLCN antibody

FLCN Rabbit Polyclonal

Cat. Num. AAA769389

• Gene Names: FLCN; BHD; FLCL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: FLCN; Polyclonal Antibody; FLCN Rabbit Polyclonal; BHD; anti-FLCN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
• Sequence Length: 579

• Applicable Applications for anti-FLCN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
• Immunogen: Folliculin
• Preparation and Storage: -20 degree C for 12 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovary tumor using MBS769389 (FLCN antibody) at dilution of 1:50)

Western Blot (WB)

(mouse thymus tissue were subjected to SDS PAGE followed by western blot with MBS769389 (FLCN antibody) at dilution of 1:300)

Testing Data

(IP result of anti-FLCN(MBS769389) with Hela cell.)

Related Product Information for anti-FLCN antibody

May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.

NCBI and Uniprot Product Information

• NCBI GI #: 22907034
• NCBI GeneID: 201163
• NCBI Accession #: NP_659434.2
• NCBI GenBank Nucleotide #: NM_144997.5
• Molecular Weight: Observed MW: 38-45kDa, 64kDa, 20-26kDa
• NCBI Official Full Name: folliculin isoform 1
• NCBI Official Synonym Full Names: folliculin
• NCBI Official Symbol: FLCN
• NCBI Official Synonym Symbols: BHD; FLCL
• NCBI Protein Information: folliculin
• UniProt Protein Name: Folliculin
• Protein Family: Folliculin
• UniProt Gene Name: FLCN
• UniProt Synonym Gene Names: BHD
• UniProt Entry Name: FLCN_HUMAN

NCBI Description

This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FLCN: a protein that is expressed in most tissues and that has no significant homology to other proteins. Involved in nutrient sensing through the AMPK and mTOR signaling pathways. Regulates lysosome function by promoting the mTORC1-dependent sequestration of transcription factor EB (TFEB). Required for the recruitment of mTORC1 to lysosomes by Rag GTPases. Amino acid depletion leads to its recruitment to the surface of lysosomes where it directly binds RagA, an interaction that is promoted by FNIP1. May be a tumor suppressor. Loss of function mutations cause Birt-Hogg-Dube syndrome (BHD), a disease characterized by fibrofolliculomas (hair follicle tumors), and predispositions for renal carcinoma, pneumothorax, and pulmonary cysts. These symptoms are similar to those that occur in TSC or tuberous sclerosis complex, suggesting the involvement in the same pathways as TSC1. Belongs to the folliculin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; lysosome; midbody; nucleus; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; protein complex binding

Biological Process: hemopoiesis; in utero embryonic development; intercellular junction assembly; negative regulation of cell growth; negative regulation of cell migration; negative regulation of protein kinase B signaling cascade; negative regulation of Rho protein signal transduction; negative regulation of TOR signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of cell adhesion; positive regulation of GTPase activity; positive regulation of protein amino acid phosphorylation; positive regulation of TOR signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of cytokinesis; regulation of histone acetylation; regulation of protein amino acid phosphorylation; regulation of TOR signaling pathway; TOR signaling pathway

Disease: Birt-hogg-dube Syndrome; Colorectal Cancer; Pneumothorax, Primary Spontaneous; Renal Cell Carcinoma, Nonpapillary