Rabbit anti-Human, Mouse FKBP1B Polyclonal Antibody | anti-FKBP1B antibody

FKBP1B, NT (E6) (h-FKBP-12, Immunophilin FKBP12.6, 12.6kD FK506-binding Protein, 12.6kD FKBP, FKBP-12.6, Rotamase, FK506-binding Protein 1B, FKBP-1B, Peptidyl-prolyl cis-trans Isomerase FKBP1B, PPIase FKBP1B, FKBP12.6, FKBP1L, FKBP9, OTK4) (FITC)

Cat. Num. AAA6475992

• Gene Names: FKBP1B; OTK4; FKBP1L; PKBP1L; PPIase; FKBP12.6
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: FKBP1B; Polyclonal Antibody; NT (E6) (h-FKBP-12; Immunophilin FKBP12.6; 12.6kD FK506-binding Protein; 12.6kD FKBP; FKBP-12.6; Rotamase; FK506-binding Protein 1B; FKBP-1B; Peptidyl-prolyl cis-trans Isomerase FKBP1B; PPIase FKBP1B; FKBP12.6; FKBP1L; FKBP9; OTK4) (FITC); anti-FKBP1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FKBP1B. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FKBP1B antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1,000; ICC/IF: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa1-30 from the N-terminal region of human FKBP1B.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-FKBP1B Antibody (N-term E6) in CEM cell line lysates (35ug/lane). FKBP1B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-FKBP1B Antibody (N-term E6) in mouse cerebellum tissue lysates (35ug/lane). FKBP1B (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FKBP1B Antibody (N-term E6) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FKBP1B antibody

Antibodies; Binding Proteins

References

1. Arakawa H., Nagase H.Biochem. Biophys. Res. Commun. 200:836-843(1994). 2. Lam E., Martin M.M., Timerman A.P.Biol. Chem. 270:26511-26522(1995). 3. Deivanayagam C.C., Carson M.Acta Crystallogr. D 56:266-271(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4758380
• NCBI GeneID: 2281
• NCBI Accession #: NP_004107
• NCBI GenBank Nucleotide #: NM_004116.4
• UniProt Accession #: P68106; Q13664; Q16645; Q53TM2; Q9BQ40
• Molecular Weight: 14.2 kDa (130aa), confirmed by MALDI-TOF
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1B isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 1B
• NCBI Official Symbol: FKBP1B
• NCBI Official Synonym Symbols: OTK4; FKBP1L; PKBP1L; PPIase; FKBP12.6
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1B
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1B
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP1B
• UniProt Synonym Gene Names: FKBP12.6; FKBP1L; FKBP9; OTK4; PPIase FKBP1B; 12.6 kDa FKBP; FKBP-12.6; FKBP-1B
• UniProt Entry Name: FKB1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are two alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FKBP1B: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the FKBP-type PPIase family. FKBP1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: sarcoplasmic reticulum membrane; membrane; cytoplasm; Z disc; cytosol

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; cyclic nucleotide binding; calcium channel inhibitor activity; ryanodine-sensitive calcium-release channel activity; receptor binding

Biological Process: positive regulation of sequestering of calcium ion; positive regulation of axon regeneration; protein maturation via protein folding; protein peptidyl-prolyl isomerization; cytosolic calcium ion homeostasis; negative regulation of heart rate; response to redox state; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; T cell proliferation; negative regulation of protein phosphatase type 2B activity; response to vitamin E; smooth muscle contraction; 'de novo' protein folding; response to hydrogen peroxide; insulin secretion; negative regulation of release of sequestered calcium ion into cytosol; action potential propagation; response to glucose stimulus; protein refolding; transmembrane transport