Rabbit FKBP12 Polyclonal Antibody | anti-FKBP1A antibody

FKBP12 Antibody

Cat. Num. AAA153690

• Gene Names: FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: FKBP12 antibody is affinity chromatography purified via peptide column.
• Synonyms: FKBP12; Polyclonal Antibody; FKBP12 Antibody; FKBP1; PKC12; PKCI2; PPIASE; FKBP-12; FKBP-1A; Peptidyl-prolyl cis-trans isomerase FKBP1A; 12 kDa FK506-binding protein; PPIase FKBP1A; FK506 binding protein 1A; 12kDa; anti-FKBP1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FKBP12 antibody is human, mouse and rat reactive.
• Purity/Purification: FKBP12 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 108

• Applicable Applications for anti-FKBP1A antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry at 5 mug/mL. For Immunoflorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12.
• Buffer: FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FKBP12 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 μg/mL.)

Related Product Information for anti-FKBP1A antibody

FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1). FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2). FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5).

NCBI and Uniprot Product Information

• NCBI GI #: 17149836
• NCBI GeneID: 2280
• NCBI Accession #: NP_463460
• NCBI GenBank Nucleotide #: NM_054014.3
• UniProt Accession #: P62942; P20071; Q4VC47; Q6FGD9; Q6LEU3; Q9H103; Q9H566; D3DVW6
• Molecular Weight: Predicted: 12 kDa; ; Observed: 12 kDa
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1A isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 1A, 12kDa
• NCBI Official Symbol: FKBP1A
• NCBI Official Synonym Symbols: FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1A; rotamase; 12 kDa FKBP; calstabin 1; calstabin-1; FKBP12-Exip3; PPIase FKBP1A; immunophilin FKBP12; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; 12 kDa FK506-binding protein; protein kinase C inhibitor 2; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1A
• Protein Family: FKBP12-associated protein
• UniProt Gene Name: FKBP1A
• UniProt Synonym Gene Names: FKBP1; FKBP12; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A
• UniProt Entry Name: FKB1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells.

Protein type: Isomerase; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; cytoplasm; nerve terminal; cytosol; Z disc

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; enzyme binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; macrolide binding; activin binding; Hsp70 protein binding; calcium channel inhibitor activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: heart morphogenesis; protein maturation via protein folding; protein peptidyl-prolyl isomerization; positive regulation of protein binding; T cell activation; protein folding; response to caffeine; T cell proliferation; regulation of protein localization; negative regulation of protein amino acid phosphorylation; muscle contraction; transforming growth factor beta receptor signaling pathway; negative regulation of release of sequestered calcium ion into cytosol; fibril organization and biogenesis; ventricular cardiac muscle morphogenesis; protein refolding; response to iron ion; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; regulation of activin receptor signaling pathway; SMAD protein complex assembly; negative regulation of protein phosphatase type 2B activity; 'de novo' protein folding; positive regulation of protein ubiquitination; release of sequestered calcium ion into cytosol