Rabbit Filamin 1 Polyclonal Antibody | anti-S2152 antibody

Filamin 1 (Phospho-S2152) Polyclonal Antibody

Cat. Num. AAA3004622

• Gene Names: FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Filamin 1; Polyclonal Antibody; Filamin 1 (Phospho-S2152) Polyclonal Antibody; FilaminA; Filamin A; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Filamin1; Non-muscle filamin; FLNA; FLN; FLN1; anti-S2152 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Filamin 1 (S2152) polyclonal antibody detects endogenous levels of Filamin 1 protein when phosphorylated at Ser2152.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2647

• Applicable Applications for anti-S2152 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Filamin 1 around the phosphorylation site of Serine 2152.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Filamin 1 (phospho-S2152) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:A549 whole cell lysate (20ug) Lane3:The Lung tissue lysate of Rat (20ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug) Lane6:SKOVCAR3 whole cell lysate (40ug) Lane7:Panc1 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-Filamin 1 (S2152) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-S2152 antibody

Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

NCBI and Uniprot Product Information

• NCBI GI #: 160420317
• NCBI GeneID: 2316
• NCBI Accession #: NP_001104026.1
• NCBI GenBank Nucleotide #: NP_001104026.1
• UniProt Accession #: P21333
• Molecular Weight: ~ 280kDa
• NCBI Official Full Name: filamin-A isoform 2
• NCBI Official Synonym Full Names: filamin A
• NCBI Official Symbol: FLNA
• NCBI Official Synonym Symbols: FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• NCBI Protein Information: filamin-A
• UniProt Protein Name: Filamin-A
• UniProt Gene Name: FLNA
• UniProt Synonym Gene Names: FLN; FLN1; FLN-A; ABP-280
• UniProt Entry Name: FLNA_HUMAN

NCBI Description

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: Rho GTPase binding; protein homodimerization activity; Ral GTPase binding; Rac GTPase binding; transcription factor binding; small GTPase binding; actin filament binding; protein binding; signal transducer activity; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding

Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; blood coagulation; negative regulation of protein catabolic process

Disease: Fg Syndrome 2; Terminal Osseous Dysplasia; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I