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Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Heart Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Brain Tissue.)
Rabbit anti-Rat Fibulin 1 (FBLN1) Polyclonal Antibody | anti-FBLN1 antibody
Polyclonal Antibody to Fibulin 1 (FBLN1)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Fibulin 1 (FBLN1); Polyclonal Antibody; Polyclonal Antibody to Fibulin 1 (FBLN1); anti-FBLN1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FBLN1. It has been selected for its ability to recognize FBLN1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-EPNL MISYQCGLVF RACCVKGQEN SDFVRGDGTD LQDPAKIPEE EEQEDPYLND RCRGGGPCKQ
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-EPNL MISYQCGLVF RACCVKGQEN SDFVRGDGTD LQDPAKIPEE EEQEDPYLND RCRGGGPCKQ
Sequence Length
683
Applicable Applications for anti-FBLN1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant FBLN1 (Glu127~Arg243) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2065974
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Heart Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Serum; Lane2: Rat Heart Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant FBLN1, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant FBLN1, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78,072 Da
NCBI Official Full Name
fibulin-1
NCBI Official Synonym Full Names
fibulin 1
NCBI Official Symbol
Fbln1
NCBI Protein Information
fibulin-1
UniProt Protein Name
Fibulin-1
UniProt Gene Name
Fbln1
Uniprot Description
Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
Research Articles on FBLN1
Similar Products
Product Notes
The FBLN1 fbln1 (Catalog #AAA2015293) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fibulin 1 (FBLN1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibulin 1 (FBLN1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FBLN1 fbln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-EPNL MISYQCGLVF RACCVKGQEN SDFVRGDGTD LQDPAKIPEE EEQEDPYLND RCRGGGPCKQ. It is sometimes possible for the material contained within the vial of "Fibulin 1 (FBLN1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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