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Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant protein.))

Rabbit anti-Rat Fibronectin (FN) Polyclonal Antibody | anti-FN antibody

Biotin-Linked Antibody to Fibronectin (FN)

Gene Names
fn1; fibronectin
Reactivity
Rat
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Fibronectin (FN); Polyclonal Antibody; Biotin-Linked Antibody to Fibronectin (FN); anti-FN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FN conjugated to biotin. It has been selected for its ability to recognize FN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence Length
2481
Applicable Applications for anti-FN antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

((Figure. Western Blot; Sample: Recombinant protein.))

Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant protein.))
Product Categories/Family for anti-FN antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
272,680 Da
NCBI Official Full Name
fibronectin
NCBI Official Synonym Full Names
fibronectin 1<
NCBI Official Symbol
fn1
NCBI Official Synonym Symbols
fibronectin
NCBI Protein Information
fibronectin; FN; gene C
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
fn1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_XENLA

Uniprot Description

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction

By similarity.

Subunit structure: Dimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends

By similarity.

Tissue specificity: In early Xenopus embryo, cellular forms of fibronectin predominate which include both extra domains. In fibronectin of embryonic and adult liver the connecting strand 3 can be either completely excluded or included.

Post-translational modification: Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.

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Product Notes

The FN fn1 (Catalog #AAA2006229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotin-Linked Antibody to Fibronectin (FN) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibronectin (FN) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FN fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin (FN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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