Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Sheep Fibroblast Growth Factor 1 Polyclonal Antibody | anti-FGF1 antibody
Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
Gene Names
FGF1; aFGF; FGF-1; HBGF-1
Reactivity
Sheep
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Fibroblast Growth Factor 1; Polyclonal Antibody; Polyclonal Antibody to Fibroblast Growth Factor 1; Acidic (FGF1); anti-FGF1 antibody
Host
Rabbit
Reactivity
Sheep
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FGF1. It has been selected for its ability to recognize FGF1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
155
Applicable Applications for anti-FGF1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
FGF1 (Ala2~Asp155)
Organism Species
Ovis aries; Ovine (Sheep)
Cross Reactivity
Sheep
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035536
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,557 Da
NCBI Official Full Name
fibroblast growth factor 1 isoform X1
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
aFGF; FGF-1; HBGF-1
NCBI Protein Information
fibroblast growth factor 1
UniProt Protein Name
Fibroblast growth factor 1
Protein Family
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
FGF-1; aFGF; HBGF-1
Uniprot Description
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.
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Product Notes
The FGF1 fgf1 (Catalog #AAA2027421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) reacts with Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Fibroblast Growth Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the FGF1 fgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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