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Western blot (WB) (Western blot analysis of extracts of various cell lines, using FHL1 antibody (MBS8500981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 90s.)

Rabbit anti-Human FHL1 Polyclonal Antibody | anti-FHL1 antibody

FHL1 Antibody

Gene Names
FHL1; KYOT; SLIM; FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
Reactivity
Human
Purity
Affinity purification
Synonyms
FHL1; Polyclonal Antibody; FHL1 Antibody; FHL-1; FHL1A; FHL1B; FLH1A; KYOT; RBMX1A; RBMX1B; SLIM; SLIM-1; SLIM1; SLIMMER; XMPMA; anti-FHL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Sequence Length
296
Application Notes
WB: 1:500-2000; IHC: 1:50-200; IF:1:50-100
Immunogen
Recombinant fusion protein containing a sequencecorresponding to amino acids 27-296 of human FHL1(NP_001153171.1)
Preparation and Storage
Storage Store at -20 degree C. Avoid freeze / thaw cycles.

Western blot (WB)

(Western blot analysis of extracts of various cell lines, using FHL1 antibody (MBS8500981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 90s.)

Western blot (WB) (Western blot analysis of extracts of various cell lines, using FHL1 antibody (MBS8500981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 90s.)
Related Product Information for anti-FHL1 antibody
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 40kDa
Calculated MW: 22kDa/31kDa/33kDa/34kDa/36kDa
NCBI Official Full Name
four and a half LIM domains protein 1 isoform 5
NCBI Official Synonym Full Names
four and a half LIM domains 1
NCBI Official Symbol
FHL1
NCBI Official Synonym Symbols
KYOT; SLIM; FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
NCBI Protein Information
four and a half LIM domains protein 1
UniProt Protein Name
Four and a half LIM domains protein 1
UniProt Gene Name
FHL1
UniProt Synonym Gene Names
SLIM1; FHL-1; SLIM; SLIM-1

NCBI Description

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

May have an involvement in muscle development or hypertrophy.

Research Articles on FHL1

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Product Notes

The FHL1 fhl1 (Catalog #AAA8500981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FHL1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. WB: 1:500-2000; IHC: 1:50-200; IF:1:50-100. Researchers should empirically determine the suitability of the FHL1 fhl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FHL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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