Rabbit anti-Human FGR Polyclonal Antibody | anti-FGR antibody

FGR, NT (Proto-oncogene c-Fgr, Tyrosine-protein Kinase Fgr, P55-FGR, SRC2) (FITC)

Cat. Num. AAA6475937

• Gene Names: FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: FGR; Polyclonal Antibody; NT (Proto-oncogene c-Fgr; Tyrosine-protein Kinase Fgr; P55-FGR; SRC2) (FITC); anti-FGR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGR.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FGR antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human FGR (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-FGR Pab is used in Western blot to detect FGR in Ramos cell lysate.)

Western Blot (WB)

(Western blot analysis of FGR (arrow) using FGR Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGR gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FGR Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FGR antibody

Antibodies; Protein Kinases

References

1. Carriero, M.V., et al., Biol. Chem. 383(1):107-113 (2002). 2. Katamine, S., et al., Mol. Cell. Biol. 8(1):259-266 (1988). 3. Nishizawa, M., et al., Mol. Cell. Biol. 6(2):511-517 (1986). 4. Cheah, M.S., et al., Nature 319(6050):238-240 (1986). 5. Tronick, S.R., et al., Proc. Natl. Acad. Sci. USA 82(19):6595-6599 (1985). 6.Oleg Tatarov, et al. Clin. Cancer Res. published 15 May 2009, 10.1158/1078-0432. CCR-08-1857. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 112382241
• NCBI GeneID: 2268
• NCBI Accession #: NP_001036194.1
• NCBI GenBank Nucleotide #: NM_001042729.1
• UniProt Accession #: P09769; Q9UIQ3; D3DPL7
• Molecular Weight: 59,479 Da
• NCBI Official Full Name: tyrosine-protein kinase Fgr
• NCBI Official Synonym Full Names: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• NCBI Official Symbol: FGR
• NCBI Official Synonym Symbols: SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• NCBI Protein Information: tyrosine-protein kinase Fgr; p55-c-fgr protein; c-fgr protooncogene; proto-oncogene c-Fgr; c-src-2 proto-oncogene; proto-oncogene tyrosine-protein kinase FGR
• UniProt Protein Name: Tyrosine-protein kinase Fgr
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: FGR
• UniProt Synonym Gene Names: SRC2
• UniProt Entry Name: FGR_HUMAN

NCBI Description

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FGR: a non-receptor tyrosine kinase of the Src family. Contains one SH2 and one SH3 domain. Beta 2 integrin signaling induces its c-Cbl-mediated ubiquitination in adherent neutrophils. An ortholog of v-fgr feline oncogene. Mildly amplified in hormone-resistant prostate cancer. Copy number either increased or decreased in other cancers.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Mitochondrial; Oncoprotein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 1p36.2-p36.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; mitochondrial inner membrane; plasma membrane; mitochondrial intermembrane space; cytosol; actin cytoskeleton

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: integrin-mediated signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; response to virus; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; immune response-regulating cell surface receptor signaling pathway; positive regulation of mast cell degranulation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of innate immune response; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; defense response to Gram-positive bacterium; innate immune response; regulation of protein kinase activity; blood coagulation; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; positive regulation of cytokine secretion