Rabbit anti-Human, Mouse FGL2 Polyclonal Antibody | anti-FGL2 antibody

FGL2, CT (FGL2, Fibroleukin, Fibrinogen-like protein 2, pT49) (MaxLight 750)

Cat. Num. AAA6299218

• Gene Names: FGL2; T49; pT49
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FGL2; Polyclonal Antibody; CT (FGL2; Fibroleukin; Fibrinogen-like protein 2; pT49) (MaxLight 750); pT49; anti-FGL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGL2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-FGL2 antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 338-367 in the C-terminal region of human FGL2.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6013554. This demonstrates that MBS6013554 detected the FGL2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6013554. This demonstrates that MBS6013554 detected the FGL2 protein (arrow).)

Testing Data

(All lanes: using MBS6013554 (1:2000) Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: KG-1 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 50kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS6013554 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FGL2 antibody

The protein encoded by this gene is a secreted protein that is similar to the beta-and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites.

Product Categories/Family for anti-FGL2 antibody

Antibodies; Fibrinogen

References

1. Liu, Y., et al. Biochem. Biophys. Res. Commun. 396(2):555-561(2010). 2. Hsieh, Y.H., et al. Bull. Math. Biol. 72(1):122-132(2010). 3. Siu, K.L., et al. J. Gen. Virol. 90 (PT 9), 2107-2113 (2009). 4. Han, M., et al. J. Biol. Chem. 283(47):32715-32729(2008). 5. Su, K., et al. World J. Gastroenterol. 14(39):5980-5989(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5730075
• NCBI GeneID: 10875
• NCBI Accession #: NP_006673.1
• NCBI GenBank Nucleotide #: NM_006682.2
• UniProt Accession #: Q14314
• Molecular Weight: 50,229 Da
• NCBI Official Full Name: fibroleukin
• NCBI Official Synonym Full Names: fibrinogen-like 2
• NCBI Official Symbol: FGL2
• NCBI Official Synonym Symbols: T49; pT49
• NCBI Protein Information: fibroleukin; fibrinogen-like protein 2
• UniProt Protein Name: Fibroleukin
• Protein Family: Fibroleukin
• UniProt Gene Name: FGL2
• UniProt Entry Name: FGL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites. [provided by RefSeq, Jul 2008]

Uniprot Description

FGL2: May play a role in physiologic lymphocyte functions at mucosal sites.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: fibrinogen complex