Rabbit anti-Human FGFR1 Polyclonal Antibody | anti-FGFR1 antibody

FGFR1 (Y653) (Basic Fibroblast Growth Factor Receptor 1, FGFR-1, bFGF-R, Proto-oncogene c-Fgr, Fms-like Tyrosine Kinase 2, FLT-2, CD331, FGFBR, FLG, FLT2) (FITC)

Cat. Num. AAA6476469

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FGFR1; Polyclonal Antibody; FGFR1 (Y653) (Basic Fibroblast Growth Factor Receptor 1; FGFR-1; bFGF-R; Proto-oncogene c-Fgr; Fms-like Tyrosine Kinase 2; FLT-2; CD331; FGFBR; FLG; FLT2) (FITC); anti-FGFR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGFR1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FGFR1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Y653 of human FGFR1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FGFR1 (arrow) using rabbit polyclonal FGFR1 Antibody (Y653) (RB11303). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-FGFR1 antibody

Antibodies; Receptors

References

1. Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003). 2. Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003). 3. Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). 4. Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003). 5. Baumann, H., et al., J. Biol. Chem. 278(18):16198-16208 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 11276087
• UniProt Accession #: P11362
• Molecular Weight: 91.867
• UniProt Protein Name: Fibroblast growth factor receptor 1
• Protein Family: FGFR1 oncogene partner
• UniProt Gene Name: FGFR1
• UniProt Synonym Gene Names: BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; FGFR-1; BFGFR; bFGF-R-1; FLT-2
• UniProt Entry Name: FGFR1_HUMAN

Uniprot Description

FGFR1: a receptor tyrosine kinase of the highly-conserved fibroblast growth factor receptor (FGFR). Binds both acidic and basic fibroblast growth factors and is involved in limb induction. Point mutations cause Pfeffer syndrome (finger and toe malformations and other skeletal errors) and dominant Kallmann syndrome 2. Stem cell leukemia lymphoma syndrome (SCLL) may be caused by a t(8;13)(p12;q12) translocation that fuses a zinc finger gene, ZNF198, to FGFR1. Various myeloproliferative disorders have been linked to translocations that fuse FGFR1 to FOP, FIM, CEP1 or the atypical kinase, Bcr. Inhibitor: SU5402. 20 isoforms of the human protein produced by alternative splicing have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; FGFR family

Chromosomal Location of Human Ortholog: 8p11.23-p11.22

Cellular Component: integral to plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; extracellular region; cytosol; nucleus; receptor complex

Molecular Function: heparin binding; identical protein binding; protein binding; fibroblast growth factor binding; protein homodimerization activity; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; ATP binding

Biological Process: paraxial mesoderm development; axon guidance; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; cell maturation; neuron migration; negative regulation of transcription from RNA polymerase II promoter; middle ear morphogenesis; protein amino acid phosphorylation; positive regulation of MAP kinase activity; sensory perception of sound; positive regulation of MAPKKK cascade; ureteric bud development; induction of an organ; regulation of cell differentiation; midbrain development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; chondrocyte differentiation; angiogenesis; skeletal development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; chordate embryonic development; transcription, DNA-dependent; in utero embryonic development; outer ear morphogenesis; MAPKKK cascade; positive regulation of cell cycle; positive regulation of phosphoinositide 3-kinase cascade; neuroblast division in the ventricular zone; mesenchymal cell differentiation; skeletal morphogenesis; insulin receptor signaling pathway; innate immune response; auditory receptor cell development; positive regulation of neuron differentiation; regulation of lateral mesodermal cell fate specification

Disease: Pfeiffer Syndrome; Hypogonadotropic Hypogonadism 2 With Or Without Anosmia; Jackson-weiss Syndrome; Trigonocephaly 1; Osteoglophonic Dysplasia