Rabbit anti-Mouse Fgf8 Polyclonal Antibody | anti-Fgf8 antibody

Fgf8, NT (Fgf8, Aigf, Fibroblast growth factor 8, Androgen-induced growth factor, Heparin-binding growth factor 8) (AP)

Cat. Num. AAA6299044

• Gene Names: Fgf8; Aigf; Fgf-8
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Fgf8; Polyclonal Antibody; NT (Fgf8; Aigf; Fibroblast growth factor 8; Androgen-induced growth factor; Heparin-binding growth factor 8) (AP); Heparin-binding growth factor 8; anti-Fgf8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Fgf8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Fgf8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of mouse Fgf8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Fgf8 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mouse Fgf8 antibody detected the Mouse Fgf8 protein (arrow).)

Related Product Information for anti-Fgf8 antibody

Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis.

Product Categories/Family for anti-Fgf8 antibody

Antibodies; Growth Factors; Cytokines

References

Yu, S., et al. Dev. Biol. 347(1):92-108(2010). Toyoda, R., et al. Development 137(20):3439-3448(2010). Leung, A.W., et al. Dev. Dyn. 239(9):2319-2329(2010). Mao, J., et al. Development 137(18):3079-3088(2010). Mitsiadis, T.A., et al. Development 137(18):3025-3035(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 261599076
• NCBI GeneID: 14179
• NCBI Accession #: NP_001159834.1
• NCBI GenBank Nucleotide #: NM_001166362.1
• UniProt Accession #: P37237
• Molecular Weight: 23,522 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform 3
• NCBI Official Synonym Full Names: fibroblast growth factor 8
• NCBI Official Symbol: Fgf8
• NCBI Official Synonym Symbols: Aigf; Fgf-8
• NCBI Protein Information: fibroblast growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: Fgf8
• UniProt Synonym Gene Names: Aigf; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_MOUSE

Uniprot Description

FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Cellular Component: external side of plasma membrane

Molecular Function: chemoattractant activity

Biological Process: adrenocorticotropin hormone secreting cell differentiation; apoptosis; blood vessel remodeling; BMP signaling pathway; cell fate commitment; cell proliferation in forebrain; central nervous system neuron development; determination of left/right symmetry; dorsal/ventral axon guidance; embryonic development ending in birth or egg hatching; embryonic heart tube development; embryonic hindlimb morphogenesis; fibroblast growth factor receptor signaling pathway; forebrain dorsal/ventral pattern formation; forebrain morphogenesis; forebrain neuron development; generation of neurons; gonad development; heart development; heart looping; heart morphogenesis; induction of an organ; inner ear morphogenesis; kidney development; limb morphogenesis; lung development; male genitalia development; MAPKKK cascade; mesodermal cell migration; midbrain-hindbrain boundary development; motor axon guidance; negative regulation of apoptosis; negative regulation of cardiac muscle development; negative regulation of neuron apoptosis; neural plate morphogenesis; otic vesicle formation; pallium development; patterning of blood vessels; pharyngeal system development; positive regulation of cell proliferation; positive regulation of mitosis; positive regulation of organ growth; regulation of odontogenesis of dentine-containing teeth; response to oxidative stress; subpallium development; telencephalon development; thyroid gland development; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway through beta-catenin