Rabbit anti-Human FGF19 Polyclonal Antibody | anti-FGF19 antibody

Anti-FGF19 Antibody

Cat. Num. AAA178817

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: FGF19; Polyclonal Antibody; Anti-FGF19 Antibody; FGF15; FGF-19; O95750; Fibroblast growth factor 19; anti-FGF19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 216

• Applicable Applications for anti-FGF19 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Western Blot: 0.1-0.5ug/ml; ELISA: 0.1-0.5ug/ml
• Notes: Tested Species: In-house tested species with positive results.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived human FGF19 recombinant protein (Position: L25-K216).
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of FGF19 expression in HELA whole cell lysates (lane 1). FGF19 at 24KD was detected using rabbit anti- FGF19 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-FGF19 antibody

Rabbit IgG polyclonal antibody for Fibroblast growth factor 19(FGF19) detection.
Background: FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. FGF19 is the orthologous protein in humans. They are often referred together as FGF15/19.

References

1. Kir, S., Beddow, S. A., Samuel, V. T., Miller, P., Previs, S. F., Suino-Powell, K., Xu, H. E., Shulman, G. I., Kliewer, S. A., Mangelsdorf, D. J.FGF19 as a postprandial, insulin-independent activator of hepatic protein and glycogen synthesis.
2. Ladher, R. K., Anakwe, K. U., Gurney, A. L., Schoenwolf, G. C., Francis-West, P. H.Identification of synergistic signals initiating inner ear development.
3. Nishimura, T., Utsunomiya, Y., Hoshikawa, M., Ohuchi, H., Itoh, N.Structure and expression of a novel human FGF, FGF-19, expressed in the fetal brain. Biochim. Biophys. Acta 1444: 148-151, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 4826726
• NCBI GeneID: 9965
• NCBI Accession #: NP_005108.1
• NCBI GenBank Nucleotide #: NM_005117.2
• UniProt Accession #: O95750
• Molecular Weight: 24,003 Da
• NCBI Official Full Name: fibroblast growth factor 19
• NCBI Official Synonym Full Names: fibroblast growth factor 19
• NCBI Official Symbol: FGF19
• NCBI Protein Information: fibroblast growth factor 19
• UniProt Protein Name: Fibroblast growth factor 19
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF19
• UniProt Synonym Gene Names: FGF-19

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: extracellular region

Molecular Function: 1-phosphatidylinositol-3-kinase activity; fibroblast growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: fibroblast growth factor receptor signaling pathway; MAPKKK cascade; nervous system development; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of glucose import; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase cascade