Rabbit anti-Mouse FGF16 Polyclonal Antibody | anti-FGF16 antibody

FGF16, NT (FGF16, Fibroblast growth factor 16) (Azide free) (HRP)

Cat. Num. AAA6298982

• Gene Names: FGF16; MF4; FGF-16
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FGF16; Polyclonal Antibody; NT (FGF16; Fibroblast growth factor 16) (Azide free) (HRP); Fibroblast growth factor 16; anti-FGF16 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FGF16 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FGF16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human FGF16.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FGF16 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FGF16 antibody detected the FGF16 protein (arrow).)

Immunohistochemistry (IHC)

(FGF16 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FGF16 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FGF16 antibody

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The rat homolog is predominantly expressed in embryonic brown adipose tissue and has significant mitogenic activity, which suggests a role in proliferation of embryonic brown adipose tissue.

Product Categories/Family for anti-FGF16 antibody

Antibodies; Growth Factors; Cytokines

References

Sofronescu, A.G., et al. Cardiovasc. Res. 87(1):102-110(2010). Moffa, A.B., et al. J. Cell. Physiol. 210(3):720-731(2007). Antoine, M., et al. Biochem. Biophys. Res. Commun. 346(1):224-233(2006). Zhang, X., et al. J. Biol. Chem. 281(23):15694-15700(2006). Popovici, C., et al. J. Biol. Chem. 279(38):40146-40152(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4503691
• NCBI GeneID: 8823
• NCBI Accession #: NP_003859.1
• NCBI GenBank Nucleotide #: NM_003868.2
• UniProt Accession #: O43320
• Molecular Weight: 23,759 Da
• NCBI Official Full Name: fibroblast growth factor 16
• NCBI Official Synonym Full Names: fibroblast growth factor 16
• NCBI Official Symbol: FGF16
• NCBI Official Synonym Symbols: MF4; FGF-16
• NCBI Protein Information: fibroblast growth factor 16
• UniProt Protein Name: Fibroblast growth factor 16
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF16
• UniProt Synonym Gene Names: FGF-16
• UniProt Entry Name: FGF16_HUMAN

Uniprot Description

FGF16: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: extracellular region; extracellular space

Molecular Function: 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell-cell signaling; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; metabolic process; organ morphogenesis; phosphoinositide-mediated signaling; regulation of endothelial cell proliferation; regulation of MAP kinase activity; regulation of phosphoinositide 3-kinase cascade; response to temperature stimulus; signal transduction

Disease: Metacarpal 4-5 Fusion