Rabbit FEN1 Polyclonal Antibody | anti-FEN1 antibody

FEN1 (Flap Endonuclease-1, FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2) (HRP)

Cat. Num. AAA6417117

• Gene Names: FEN1; MF1; RAD2; FEN-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: FEN1; Polyclonal Antibody; FEN1 (Flap Endonuclease-1; FEN-1; Flap Structure-specific Endonuclease 1; MF1; Maturation Factor-1; RAD2) (HRP); anti-FEN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes FEN1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-FEN1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human FEN1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FEN1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectal cancer using FEN1 antibody at dilution of 1:200 (400x lens).)

Product Categories/Family for anti-FEN1 antibody

Antibodies; Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 4758356
• NCBI GeneID: 2237
• NCBI Accession #: NP_004102.1
• NCBI GenBank Nucleotide #: NM_004111.5
• UniProt Accession #: P39748; AAB32522
• Molecular Weight: 42,593 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure-specific endonuclease 1
• NCBI Official Symbol: FEN1
• NCBI Official Synonym Symbols: MF1; RAD2; FEN-1
• NCBI Protein Information: flap endonuclease 1; DNase IV; maturation factor 1; maturation factor-1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: FEN1
• UniProt Synonym Gene Names: RAD2; FEN-1; MF1; hFEN-1
• UniProt Entry Name: FEN1_HUMAN

NCBI Description

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Uniprot Description

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: Ribonuclease; DNA repair, damage; Nucleolus; EC 3.1.-.-; Nuclear receptor co-regulator; DNA-binding; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; membrane; mitochondrion; plasma membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; DNA binding; 5'-flap endonuclease activity; manganese ion binding; endonuclease activity; exonuclease activity; double-stranded DNA binding; damaged DNA binding; magnesium ion binding; ribonuclease H activity; double-stranded DNA specific exodeoxyribonuclease activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication, removal of RNA primer; UV protection; base-excision repair; double-strand break repair; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA catabolic process, exonucleolytic; DNA repair; telomere maintenance; DNA catabolic process, endonucleolytic; memory