Rabbit FECH Polyclonal Antibody | anti-FECH antibody

FECH Polyclonal Antibody

Cat. Num. AAA9128274

• Gene Names: FECH; EPP; FCE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: FECH; Polyclonal Antibody; FECH Polyclonal Antibody; anti-FECH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 423

• Applicable Applications for anti-FECH antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human FECH
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FECH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using FECH antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat heart using FECH antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-FECH antibody

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.

NCBI and Uniprot Product Information

• NCBI GI #: 60499021
• NCBI GeneID: 2235
• NCBI Accession #: NP_000131.2
• NCBI GenBank Nucleotide #: NM_000140.3
• UniProt Accession #: P22830; Q8IXN1; Q8NAN0; A8KA72
• Molecular Weight: 48kDa
• NCBI Official Full Name: ferrochelatase, mitochondrial isoform b
• NCBI Official Synonym Full Names: ferrochelatase
• NCBI Official Symbol: FECH
• NCBI Official Synonym Symbols: EPP; FCE
• NCBI Protein Information: ferrochelatase, mitochondrial
• UniProt Protein Name: Ferrochelatase, mitochondrial
• UniProt Gene Name: FECH
• UniProt Entry Name: HEMH_HUMAN

NCBI Description

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010]

Uniprot Description

FECH: Catalyzes the ferrous insertion into protoporphyrin IX. Defects in FECH are the cause of erythropoietic protoporphyria (EPP). Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. EPP is a form of porphyria marked by excessive protoporphyrin in erythrocytes, plasma, liver and feces, and by widely varying photosensitive skin changes ranging from a burning or pruritic sensation to erythema, edema and wheals. Belongs to the ferrochelatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Mitochondrial; EC 4.99.1.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; protein binding; iron-responsive element binding; ferrochelatase activity; ferrous iron binding; heme binding

Biological Process: response to drug; response to light stimulus; cholesterol metabolic process; generation of precursor metabolites and energy; response to arsenic; response to insecticide; detection of UV; iron ion homeostasis; response to ethanol; regulation of gene expression; protoporphyrinogen IX metabolic process; regulation of hemoglobin biosynthetic process; porphyrin metabolic process; response to methylmercury; response to lead ion; erythrocyte differentiation; heme biosynthetic process

Disease: Protoporphyria, Erythropoietic