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Western Blot (WB) (Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6003686. FCGR1B (arrow) was detected usingMBS6003686.)

Rabbit anti-Human FCGR1B Polyclonal Antibody | anti-FCGR1B antibody

FCGR1B, CT (FCGR1B, IGFRB, High affinity immunoglobulin gamma Fc receptor IB, Fc-gamma RIB) (MaxLight 490)

Gene Names
FCGR1B; CD64b; IGFRB
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
FCGR1B; Polyclonal Antibody; CT (FCGR1B; IGFRB; High affinity immunoglobulin gamma Fc receptor IB; Fc-gamma RIB) (MaxLight 490); Fc-gamma RIB; anti-FCGR1B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FCGR1B.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-FCGR1B antibody
Western Blot (WB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 248-276 in the C-terminal region of human FCGR1B.
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6003686. FCGR1B (arrow) was detected usingMBS6003686.)

Western Blot (WB) (Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6003686. FCGR1B (arrow) was detected usingMBS6003686.)

Testing Data

(All lanes: MBS6003686 (1:500) Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 33kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (All lanes: MBS6003686 (1:500) Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 33kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS6003686 (1:500) Lane 1: CCRF-CEM whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 33kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (All lanes: MBS6003686 (1:500) Lane 1: CCRF-CEM whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 33kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FCGR1B antibody
FCGR1B may bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. This protein may function in the humoral immune response.
Product Categories/Family for anti-FCGR1B antibody
References
1. Thomas, G., et.al., Nat. Genet. 41 (5), 579-584 (2009). 2. Kuwano, Y., et.al., Arch. Dermatol. Res. 298 (10), 493-498 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,232 Da
NCBI Official Full Name
high affinity immunoglobulin gamma Fc receptor IB isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, high affinity Ib, receptor (CD64)
NCBI Official Symbol
FCGR1B
NCBI Official Synonym Symbols
CD64b; IGFRB
NCBI Protein Information
high affinity immunoglobulin gamma Fc receptor IB; fcRIB; hFcgammaRIB; fc-gamma RIB; igG Fc receptor IB; Fc-gamma receptor I B2
UniProt Protein Name
High affinity immunoglobulin gamma Fc receptor IB
UniProt Gene Name
FCGR1B
UniProt Synonym Gene Names
IGFRB; IgG Fc receptor IB; FcRIB; hFcgammaRIB
UniProt Entry Name
FCGRB_HUMAN

NCBI Description

Three distinct, but closely related classes of receptors that bind the Fc portion of IgG have been identified (Fcgamma RI, II and III). The FcgammaRI family consists of three closely related genes termed A, B, and C. This gene encodes the low affinity FcgammaRIB receptor that may play an important role in humoral immune response. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

FCGR1B: May bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. May function in the humoral immune response. Belongs to the immunoglobulin superfamily. FCGR1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p11.2

Cellular Component: early endosome membrane; plasma membrane; integral to membrane

Molecular Function: IgG binding; immunoglobulin receptor activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Research Articles on FCGR1B

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Product Notes

The FCGR1B fcgr1b (Catalog #AAA6298621) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FCGR1B, CT (FCGR1B, IGFRB, High affinity immunoglobulin gamma Fc receptor IB, Fc-gamma RIB) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FCGR1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FCGR1B fcgr1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FCGR1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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