Rabbit FBXW11 Polyclonal Antibody | anti-FBXW11 antibody

FBXW11 Antibody

Cat. Num. AAA9611760

• Gene Names: FBXW11; Hos; BTRC2; FBW1B; Fbw11; BTRCP2; FBXW1B
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FBXW11; Polyclonal Antibody; FBXW11 Antibody; beta Transducin Repeat Containing Protein 2; BTRC2; BTRCP2; F-box and WD repeats protein beta-TrCP2; F-box and WD-40 Domain Protein 1B; F-box Protein FBW1B; F-box/WD repeat-containing protein 11; F-box/WD repeat-containing protein 1B; FBW1B; FBW1B_HUMAN; FBXW1B; Homologous to Slimb protein; HOS; anti-FBXW11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FBXW11 Antibody detects endogenous levels of FBXW11.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 542

• Applicable Applications for anti-FBXW11 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human FBXW11.
• Tissue Specificity: Expression is negatively regulated by Wnt/beta-catenin pathway.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from MGC-803 cells, using FBXW11 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-FBXW11 antibody

Description: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of phosphorylated CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. SCF(FBXW11) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25503564). Involved in the oxidative stress-induced a ubiquitin-mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and phosphorylated PER2. SCF(FBXW11) mediates the ubiquitination of CYTH1, and probably CYTH2 (PubMed:29420262).

Subunit Structure: Self-associates. Component of the SCF(FBXW11) complex formed of CUL1, SKP1, RBX1 and a FBXW11 dimer. Interacts with BTRC, BST2, PER1, RCAN1 and USP47. Interacts with phosphorylated ubiquitination substrates CTNNB1, NFKBIA, IFNAR1, PER1 and PER2; the interaction requires the phosphorylation of the two serine residues in the substrates' destruction motif D-S-G-X(2,3,4)-S. Interacts with TRIM21. Interacts with PER3. Interacts with phosphorylated ubiquitination substrate CEP68 (PubMed:25503564). Interacts with INAVA (PubMed:29420262).

Similarity: The N-terminal D domain mediates homodimerization.

NCBI and Uniprot Product Information

• NCBI GI #: 48928050
• NCBI GeneID: 23291
• NCBI Accession #: NP_036432
• NCBI GenBank Nucleotide #: NP_036432.2
• UniProt Accession #: Q9UKB1
• Molecular Weight: Observed: 61 kDa; Predicted: 63 kDa
• NCBI Official Full Name: F-box/WD repeat-containing protein 11 isoform C
• NCBI Official Synonym Full Names: F-box and WD repeat domain containing 11
• NCBI Official Symbol: FBXW11
• NCBI Official Synonym Symbols: Hos; BTRC2; FBW1B; Fbw11; BTRCP2; FBXW1B
• NCBI Protein Information: F-box/WD repeat-containing protein 11
• UniProt Protein Name: F-box/WD repeat-containing protein 11
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: FBXW11
• UniProt Synonym Gene Names: BTRCP2; FBW1B; FBXW1B; KIAA0696; HOS
• UniProt Entry Name: FBW1B_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. [provided by RefSeq, Jul 2008]