Rabbit anti-Human FBXO44 Polyclonal Antibody | anti-FBXO44 antibody

FBXO44, NT (FBXO44, FBG3, FBX30, FBX44, FBX6A, FBXO6A, F-box only protein 44, F-box protein FBX30, F-box/G-domain protein 3) (Azide free) (HRP)

Cat. Num. AAA6298454

• Gene Names: FBXO44; FBG3; FBX30; FBX6A; Fbx44; Fbxo6a
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO44; Polyclonal Antibody; NT (FBXO44; FBG3; FBX30; FBX44; FBX6A; FBXO6A; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3) (Azide free) (HRP); F-box/G-domain protein 3; anti-FBXO44 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FBXO44 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FBXO44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FBXO44.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO44 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBXO44 Antibody detected the FBXO44 protein (arrow).)

Product Categories/Family for anti-FBXO44 antibody

Antibodies; Ubiquitin Ligases

References

Kumanomidou, T., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 1), 95-98 (2010): Glenn, K.A., et al. J. Biol. Chem. 283(19):12717-12729(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Jin, J., et al. Genes Dev. 18(21):2573-2580(2004). Ilyin, G.P., et al. Gene 296 (1-2), 11-20 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 62388868
• NCBI GeneID: 93611
• NCBI Accession #: NP_001014765.1
• NCBI GenBank Nucleotide #: NM_001014765.1
• UniProt Accession #: Q9H4M3; Q5TGX2; Q5TGX4; Q5TGX5; Q68DJ9; Q8WWY2; B3KNZ2; B7Z743
• Molecular Weight: 29,747 Da
• NCBI Official Full Name: F-box only protein 44 isoform 1
• NCBI Official Synonym Full Names: F-box protein 44
• NCBI Official Symbol: FBXO44
• NCBI Official Synonym Symbols: FBG3; FBX30; FBX6A; Fbx44; Fbxo6a
• NCBI Protein Information: F-box only protein 44; F-box gene 3; F-box protein FBX30; F-box/G-domain protein 3
• UniProt Protein Name: F-box only protein 44
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO44
• UniProt Synonym Gene Names: FBG3; FBX30; FBX44; FBX6A; FBXO6A
• UniProt Entry Name: FBX44_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Four alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO44: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: SCF ubiquitin ligase complex

Molecular Function: protein binding