Western Blot (WB)
(Western Blot analysis of FBXO42 expression in transfected 293T cell line by FBXO42 polyclonal antibody. Lane 1: FBXO42 transfected lysate (77.8kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human FBXO42 Polyclonal Antibody | anti-FBXO42 antibody
FBXO42 (F-box Only Protein 42, F-box Protein 42, FBX42, Just One F-box and Kelch Domain-containing Protein, JFK, KIAA1332)
Gene Names
FBXO42; JFK; Fbx42
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
FBXO42; Polyclonal Antibody; FBXO42 (F-box Only Protein 42; F-box Protein 42; FBX42; Just One F-box and Kelch Domain-containing Protein; JFK; KIAA1332); Anti -FBXO42 (F-box Only Protein 42; anti-FBXO42 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FBXO42.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MASSSDSEDDSFMAVDQEETVLEGTMDQDEEPHPVLEAEETRHNRSMSELPEEVLEYILSFLSPYQEHKTAALVCKQWYRLIKGVAHQCYHGFMKAVQEGNIQWESRTYPYPGTPITQRFSHSACYYDANQSMYVFGGCTQSSCNAAFNDLWRLDLNSKEWIRPLASGSYPSPKAGATLVVYKDLLVLFGGWTRPSPYPLHQPERFFDEIHTYSPSKNWWNCIVTTHGPPPMAGHSSCVIDDKMIVFGGSLGSRQMSNDVWVLDLEQWAWSKPNISGPSPHPRGGQSQIVIDDATILILGGCGGPNALFKDAWLLHMHSGPWAWQPLKVENEEHGAPELWCHPACRVGQCVVVFSQAPSGRAPLSPSLNSRPSPISATPPALVPETREYRSQSPVRSMDEAPCVNGRWGTLRPRAQRQTPSGSREGSLSPARGDGSPILNGGSLSPGTAAVGGSLDSPVQAISPSTPSAPEGYDLKIGLSLAPRRGSLPDQKDLRLGSIDLNWDLKPASSSNPMDGMDNRTVGGSMRHPPEQTNGVHTPPHVASALAGAVSPGALRRSLEAIKAMSSKGPSASAALSPPLGSSPGSPGSQSLSSGETVPIPRPGPAQGDGHSLPPIARRLGHHPPQSLNVGKPLYQSMNCKPMQMYVLDIKDTKEKGRVKWKVFNSSSVVGPPETSLHTVVQGRGELIIFGGLMDKKQNVKYYPKTNALYFVRAKR
Applicable Applications for anti-FBXO42 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human FBXO42, aa1-716 (AAH43410.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of FBXO42 expression in transfected 293T cell line by FBXO42 polyclonal antibody. Lane 1: FBXO42 transfected lysate (77.8kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of FBXO42 expression in transfected 293T cell line by FBXO42 polyclonal antibody. Lane 1: FBXO42 transfected lysate (77.8kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-FBXO42 antibody
Members of the F-box protein family, such as FBXO42, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
Product Categories/Family for anti-FBXO42 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
77,839 Da
NCBI Official Full Name
FBXO42 protein, partial
NCBI Official Synonym Full Names
F-box protein 42
NCBI Official Symbol
FBXO42
NCBI Official Synonym Symbols
JFK; Fbx42
NCBI Protein Information
F-box only protein 42; just one F-box and Kelch domain-containing protein
UniProt Protein Name
F-box only protein 42
Protein Family
UniProt Gene Name
FBXO42
UniProt Synonym Gene Names
FBX42; JFK; KIAA1332
UniProt Entry Name
FBX42_HUMAN
NCBI Description
Members of the F-box protein family, such as FBXO42, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (SKP1A; MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Dec 2010]
Uniprot Description
FBXO42: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complex. Specifically recognizes p53/TP53, promoting its ubiquitination and degradation.
Chromosomal Location of Human Ortholog: 1p36.23-p36.11
Research Articles on FBXO42
Similar Products
Product Notes
The FBXO42 fbxo42 (Catalog #AAA6000558) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXO42 (F-box Only Protein 42, F-box Protein 42, FBX42, Just One F-box and Kelch Domain-containing Protein, JFK, KIAA1332) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FBXO42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the FBXO42 fbxo42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASSSDSEDD SFMAVDQEET VLEGTMDQDE EPHPVLEAEE TRHNRSMSEL PEEVLEYILS FLSPYQEHKT AALVCKQWYR LIKGVAHQCY HGFMKAVQEG NIQWESRTYP YPGTPITQRF SHSACYYDAN QSMYVFGGCT QSSCNAAFND LWRLDLNSKE WIRPLASGSY PSPKAGATLV VYKDLLVLFG GWTRPSPYPL HQPERFFDEI HTYSPSKNWW NCIVTTHGPP PMAGHSSCVI DDKMIVFGGS LGSRQMSNDV WVLDLEQWAW SKPNISGPSP HPRGGQSQIV IDDATILILG GCGGPNALFK DAWLLHMHSG PWAWQPLKVE NEEHGAPELW CHPACRVGQC VVVFSQAPSG RAPLSPSLNS RPSPISATPP ALVPETREYR SQSPVRSMDE APCVNGRWGT LRPRAQRQTP SGSREGSLSP ARGDGSPILN GGSLSPGTAA VGGSLDSPVQ AISPSTPSAP EGYDLKIGLS LAPRRGSLPD QKDLRLGSID LNWDLKPASS SNPMDGMDNR TVGGSMRHPP EQTNGVHTPP HVASALAGAV SPGALRRSLE AIKAMSSKGP SASAALSPPL GSSPGSPGSQ SLSSGETVPI PRPGPAQGDG HSLPPIARRL GHHPPQSLNV GKPLYQSMNC KPMQMYVLDI KDTKEKGRVK WKVFNSSSVV GPPETSLHTV VQGRGELIIF GGLMDKKQNV KYYPKTNALY FVRAKR. It is sometimes possible for the material contained within the vial of "FBXO42, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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