Rabbit anti-Human FBXO11 Polyclonal Antibody | anti-FBXO11 antibody

FBXO11, NT (FBXO11, FBX11, VIT1, F-box only protein 11, Vitiligo-associated protein 1) (AP)

Cat. Num. AAA6298307

• Gene Names: FBXO11; UBR6; VIT1; FBX11; PRMT9; UG063H01
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO11; Polyclonal Antibody; NT (FBXO11; FBX11; VIT1; F-box only protein 11; Vitiligo-associated protein 1) (AP); Vitiligo-associated protein 1; anti-FBXO11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FBXO11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FBXO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human FBXO11.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FBXO11 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the FBXO11 antibody detected the FBXO11 protein (arrow).)

Product Categories/Family for anti-FBXO11 antibody

Antibodies; Ubiquitin Ligases

References

Guan, C., et al. Int. J. Mol. Med. 26(1):57-65(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Guan, C.P., et al. Zhonghua Yi Xue Za Zhi 90(16):1126-1130(2010). Abida, W.M., et al. J. Biol. Chem. 282(3):1797-1804(2007). Segade, F., et al. Arch. Otolaryngol. Head Neck Surg. 132(7):729-733(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 298231105
• NCBI GeneID: 80204
• NCBI Accession #: NP_001177203.1
• NCBI GenBank Nucleotide #: NM_001190274.1
• UniProt Accession #: Q86XK2; Q52ZP1; Q53EP7; Q53RT5; Q8IXG3; Q96E90; Q9H6V8; Q9H9L1; Q9NR14; Q9UFK1; Q9UHI1; A1L491
• Molecular Weight: 103,585 Da
• NCBI Official Full Name: F-box only protein 11 isoform 4
• NCBI Official Synonym Full Names: F-box protein 11
• NCBI Official Symbol: FBXO11
• NCBI Official Synonym Symbols: UBR6; VIT1; FBX11; PRMT9; UG063H01
• NCBI Protein Information: F-box only protein 11; vitiligo-associated protein 1; vitiligo-associated protein VIT-1; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6
• UniProt Protein Name: F-box only protein 11
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO11
• UniProt Synonym Gene Names: FBX11; PRMT9; VIT1; VIT-1
• UniProt Entry Name: FBX11_HUMAN

Uniprot Description

PRMT9: Substrate recognition component of the a (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of TP53. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cytoplasm; nucleolus; chromosome; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein-arginine N-methyltransferase activity; zinc ion binding; ubiquitin-protein ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; sensory perception of sound; protein modification process; protein ubiquitination; peptidyl-arginine N-methylation