Rabbit anti-Human FBXL14 Polyclonal Antibody | anti-FBXL14 antibody

FBXL14, CT (FBXL14, FBL14, F-box/LRR-repeat protein 14, F-box and leucine-rich repeat protein 14) (Azide free) (HRP)

Cat. Num. AAA6298245

• Gene Names: FBXL14; Fbl14
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXL14; Polyclonal Antibody; CT (FBXL14; FBL14; F-box/LRR-repeat protein 14; F-box and leucine-rich repeat protein 14) (Azide free) (HRP); F-box and leucine-rich repeat protein 14; anti-FBXL14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FBXL14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FBXL14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-417 amino acids from the C-terminal region of human FBXL14.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXL14 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).)

Flow Cytometry (FC/FACS)

(FBXL14 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FBXL14 antibody

FBXL14 is members of the F-box protein family, such as FBXL14, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.

Product Categories/Family for anti-FBXL14 antibody

Antibodies; Ubiquitin Ligases

References

Vinas-Castells, R., et al. J. Biol. Chem. 285(6):3794-3805(2010). Jin, J., et al. Genes Dev. 18(21):2573-2580(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 22748931
• NCBI GeneID: 144699
• NCBI Accession #: NP_689654.1
• NCBI GenBank Nucleotide #: NM_152441.2
• UniProt Accession #: Q8N1E6
• NCBI Official Full Name: F-box/LRR-repeat protein 14
• NCBI Official Synonym Full Names: F-box and leucine rich repeat protein 14
• NCBI Official Symbol: FBXL14
• NCBI Official Synonym Symbols: Fbl14
• NCBI Protein Information: F-box/LRR-repeat protein 14
• UniProt Protein Name: F-box/LRR-repeat protein 14
• UniProt Gene Name: FBXL14
• UniProt Synonym Gene Names: FBL14
• UniProt Entry Name: FXL14_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXL14, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]