Rabbit FBL Polyclonal Antibody | anti-FBL antibody

FBL Antibody

Cat. Num. AAA9408856

• Gene Names: FBL; FIB; FLRN; RNU3IP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: FBL; Polyclonal Antibody; FBL Antibody; FIB; FLRN; RNU3IP1; anti-FBL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total FBL protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 321

• Applicable Applications for anti-FBL antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human FBL.
• Target Name: FBL
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FBL antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast cancer using FBL antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain using FBL antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using FBL antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-FBL antibody

Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).

Product Categories/Family for anti-FBL antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12056465
• NCBI GeneID: 2091
• NCBI Accession #: NP_001427.2
• NCBI GenBank Nucleotide #: NM_001436.3
• UniProt Accession #: P22087; O75259; Q6IAT5; Q9UPI6; B5BUE8
• Molecular Weight: 33,784 Da
• NCBI Official Full Name: rRNA 2'-O-methyltransferase fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: Nucleolus; EC 2.1.1.-; RNA processing; Methyltransferase; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; tRNA processing; snoRNA metabolic process; rRNA processing