Rabbit anti-Human FAU Polyclonal Antibody | anti-FAU antibody

FAU, CT (Ubiquitin-like Protein FUBI) (PE)

Cat. Num. AAA6475709

• Gene Names: FAU; S30; FAU1; Fub1; Fubi; asr1; RPS30; MNSFbeta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: FAU; Polyclonal Antibody; CT (Ubiquitin-like Protein FUBI) (PE); anti-FAU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FAU.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FAU antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human FUBI (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-FUBI C-term Pab in Hela and T47D cell line lysates. FUBI(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-hFUBI C-term Pab in 293 cell line lysates transiently transfected with the FAU gene (2ug/lane). FUBI(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-FAU antibody

Antibodies; Ubiquitin

References

1. Rossman, T.G., et al., Oncogene 22(12):1817-1821 (2003). 2. Kenmochi, N., et al., Genome Res. 8(5):509-523 (1998). 3. Vladimirov, S.N., et al., Eur. J. Biochem. 239(1):144-149 (1996). 4. Kas, K., et al., Genomics 17(2):387-392 (1993). 5. Michiels, L., et al., Oncogene 8(9):2537-2546 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4503659
• NCBI GeneID: 2197
• NCBI Accession #: NP_001988
• NCBI GenBank Nucleotide #: NM_001997
• UniProt Accession #: P62861; P35544
• Molecular Weight: 14kDa
• NCBI Official Full Name: ubiquitin-like protein fubi and ribosomal protein S30
• NCBI Official Synonym Full Names: FAU ubiquitin like and ribosomal protein S30 fusion
• NCBI Official Symbol: FAU
• NCBI Official Synonym Symbols: S30; FAU1; Fub1; Fubi; asr1; RPS30; MNSFbeta
• NCBI Protein Information: ubiquitin-like protein fubi and ribosomal protein S30
• UniProt Protein Name: 40S ribosomal protein S30
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: FAU
• UniProt Entry Name: RS30_HUMAN

NCBI Description

This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome and displays antimicrobial activity. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq, Nov 2014]

Uniprot Description

RPS30: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq, Jul 2008]

Protein type: Apoptosis; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytosol; extracellular space; small ribosomal subunit

Molecular Function: RNA binding; structural constituent of ribosome

Biological Process: antibacterial humoral response; cellular protein metabolic process; defense response to Gram-positive bacterium; gene expression; innate immune response in mucosa; mRNA catabolic process, nonsense-mediated decay; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription