Rabbit anti-Human FANCD2 Polyclonal Antibody | anti-FANCD2 antibody

FANCD2 Polyclonal Antibody

Cat. Num. AAA3008072

• Gene Names: FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FANCD2; Polyclonal Antibody; FANCD2 Polyclonal Antibody; Fanconi anemia group D2 protein; FACD2; FACD; anti-FANCD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FANCD2 polyclonal antibody detects endogenous levels of FANCD2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1451

• Applicable Applications for anti-FANCD2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100
• Immunogen: Recombinant full length Human FANCD2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of FANCD2 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cells, using FANCD2 polyclonal antibody)

Related Product Information for anti-FANCD2 antibody

Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci. In male meiosis, FANCD2 also co-localizes with BRCA1 at synaptonemal complexes. The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1, 451-amino acid nuclear protein that exists as 2 protein isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 66528888
• NCBI GeneID: 2177
• NCBI Accession #: NP_001018125.1
• NCBI GenBank Nucleotide #: NP_001018125.1
• UniProt Accession #: Q9BXW9
• Molecular Weight: ~ 166kDa
• NCBI Official Full Name: Fanconi anemia group D2 protein isoform b
• NCBI Official Synonym Full Names: FA complementation group D2
• NCBI Official Symbol: FANCD2
• NCBI Official Synonym Symbols: FA4; FAD; FACD; FAD2; FA-D2; FANCD
• NCBI Protein Information: Fanconi anemia group D2 protein
• UniProt Protein Name: Fanconi anemia group D2 protein
• Protein Family: FANCD2 opposite strand protein
• UniProt Gene Name: FANCD2
• UniProt Synonym Gene Names: FACD; Protein FACD2
• UniProt Entry Name: FACD2_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

FANCD2: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1 and BLM. Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3. The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage. Interacts with DCLRE1B/Apollo. Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas. Highly expressed in testis, where expression is restricted to maturing spermatocytes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 3p26

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; condensed chromosome

Molecular Function: protein binding

Biological Process: gamete generation; synapsis; response to gamma radiation; DNA repair

Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D2