Western Blot (WB)
(Anti-FAM125A Antibody (N-Term) at 1:2000 dilution + PC-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human FAM125A Polyclonal Antibody | anti-FAM125A antibody
FAM125A Antibody (N-Term)
Gene Names
MVB12A; CFBP; FAM125A
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
FAM125A; Polyclonal Antibody; FAM125A Antibody (N-Term); Multivesicular body subunit 12A; CIN85/CD2AP family-binding protein; ESCRT-I complex subunit MVB12A; Protein FAM125A; MVB12A; CFBP; anti-FAM125A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This FAM125A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from human FAM125A.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
273
Applicable Applications for anti-FAM125A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Cytoplasm. Nucleus. Endosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Late endosome membrane; Peripheral membrane protein. Note=Colocalizes with F-actin. Some fraction may be nuclear
Tissue Location
Ubiquitously expressed except in skeletal muscle.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-FAM125A Antibody (N-Term) at 1:2000 dilution + PC-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-FAM125A Antibody (N-Term) at 1:2000 dilution + PC-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FAM125A antibody
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down-regulation of EGF receptor.
Product Categories/Family for anti-FAM125A antibody
References
Konishi H.,et al.J. Biol. Chem. 281:28919-28931 (2006). Beausoleil S.A.,et al.Nat. Biotechnol. 24:1285-1292 (2006). Morita E.,et al.Cell Host Microbe 2:41-53 (2007). Morita E.,et al.EMBO J. 26:4215-4227 (2007). Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28783 Da
NCBI Official Full Name
multivesicular body subunit 12A isoform 1
NCBI Official Synonym Full Names
multivesicular body subunit 12A
NCBI Official Symbol
MVB12A
NCBI Official Synonym Symbols
CFBP; FAM125A
NCBI Protein Information
multivesicular body subunit 12A
UniProt Protein Name
Multivesicular body subunit 12A
UniProt Gene Name
MVB12A
UniProt Synonym Gene Names
CFBP; FAM125A
Uniprot Description
FAM125A: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down- regulation of EGF receptor. Belongs to the FAM125 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: centrosome; cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; vesicle
Molecular Function: lipid binding; protein binding; ubiquitin binding
Biological Process: autophagy; positive regulation of viral reproduction; regulation of epidermal growth factor receptor signaling pathway; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; virus maturation
Research Articles on FAM125A
Similar Products
Product Notes
The FAM125A mvb12a (Catalog #AAA9231055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAM125A Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM125A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the FAM125A mvb12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM125A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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