Rabbit FAK Polyclonal Antibody | anti-FAK antibody

FAK (phospho-Tyr576/Tyr577) Antibody

Cat. Num. AAA9411727

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: FAK; Polyclonal Antibody; FAK (phospho-Tyr576/Tyr577) Antibody; FADK 1; FAK1; PTK2; anti-FAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1065

• Applicable Applications for anti-FAK antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
• Modification: Phospho-Tyr576/Tyr577
• Target Name: FAK
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK(phospho-Tyr576/Tyr577) Antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using FAK(phospho-Tyr576/Tyr577) Antibody.)

Related Product Information for anti-FAK antibody

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Product Categories/Family for anti-FAK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 313851044
• NCBI GeneID: 5747
• NCBI Accession #: NP_001186578.1
• NCBI GenBank Nucleotide #: NM_001199649.1
• UniProt Accession #: Q05397; Q14291; Q8IYN9; Q9UD85; B4E2N6; F5H4S4; J3QT16
• Molecular Weight: 114,253 Da
• NCBI Official Full Name: focal adhesion kinase 1 isoform c
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1
• UniProt Protein Name: Focal adhesion kinase 1
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; PPP1R71
• UniProt Entry Name: FAK1_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Fak family

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; cytoskeleton; lamellipodium; apical plasma membrane; cytoplasm; stress fiber; plasma membrane; microtubule organizing center; cell cortex; cytosol; nucleus

Molecular Function: JUN kinase binding; signal transducer activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity

Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; establishment of nucleus localization; apoptosis; protein amino acid autophosphorylation; neuron migration; cell motility involved in cell locomotion; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; cell structure disassembly during apoptosis; placenta development; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; platelet activation; central nervous system neuron axonogenesis; regulation of osteoblast differentiation; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; endothelial cell migration; innate immune response; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration; regulation of cytoskeleton organization and biogenesis; negative regulation of apoptosis