Rabbit FAK Polyclonal Antibody | anti-FAK antibody

FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) (FITC)

Cat. Num. AAA6475555

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Reactivity: Human, Hamster, Mouse, Rat
• Applications: Immunoprecipitation, Immunocytochemistry, Western Blot
• Purity: Purified by Protein A Chromatography
• Synonyms: FAK; Polyclonal Antibody; FAK (Focal Adhesion Associated Protein Tyrosine Kinase; BC3) (FITC); anti-FAK antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes and is specific for human p125 FAK. Does not crossreact with Pyk2. Species Crossreactivity: mouse, rat and hamster. Does not crossreact wirh avian.
• Purity/Purification: Purified by Protein A Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FAK antibody: Immunoprecipitation (IP), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: IP: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate.; ICC: 10ug/ml shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.; Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
• Immunogen: pGEX-derived fusion protein containing aa748-1053 of human FAK.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot on NIH 3T3 cell lysate probed with aF0019-56 (1ug/ml).)

Product Categories/Family for anti-FAK antibody

Antibodies; Tyrosine Kinases

References

Kanner, S., et al., Proc. Natl. Acad. Sci. USA 87: 3328, 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 313851044
• NCBI GeneID: 5747
• NCBI Accession #: NP_001186578.1
• NCBI GenBank Nucleotide #: NM_001199649.1
• UniProt Accession #: Q05397; L13616
• Molecular Weight: 119,233 Da
• NCBI Official Full Name: focal adhesion kinase 1 isoform c
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1; FADK 1; PTK2 protein tyrosine kinase 2; FAK-related non-kinase polypeptide; focal adhesion kinase-related nonkinase; protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71
• UniProt Protein Name: Focal adhesion kinase 1
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; PPP1R71
• UniProt Entry Name: FAK1_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fak family

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; cytoskeleton; lamellipodium; cytoplasm; apical plasma membrane; stress fiber; plasma membrane; microtubule organizing center; cell cortex; cytosol; nucleus

Molecular Function: JUN kinase binding; protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity

Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; establishment of nucleus localization; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; cell motility involved in cell locomotion; neuron migration; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; cell structure disassembly during apoptosis; placenta development; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; regulation of osteoblast differentiation; central nervous system neuron axonogenesis; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; endothelial cell migration; innate immune response; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration; regulation of cytoskeleton organization and biogenesis; negative regulation of apoptosis