Rabbit FADS3 Polyclonal Antibody | anti-FADS3 antibody

Anti-FADS3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102584

• Gene Names: FADS3; CYB5RP; LLCDL3
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: FADS3; Polyclonal Antibody; Anti-FADS3 Antibody; Rabbit Polyclonal; FADS3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CYB5RP Antibody; Anti-LLCDL3 Antibody; anti-FADS3 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FADS3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FADS3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human FADS3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human FADS3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FADS3 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:51 kDa Observed band size:51 kDa)

Immunoprecipitation (IP)

(C9orf85 was immunoprecipitated using: Lane A:0.5 mg Rat kidney Whole Cell Lysate 4 uL anti-C9orf85 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-C9orf85 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size :54 kDa)

Related Product Information for anti-FADS3 antibody

Fatty acid desaturase 3 (FADS3) belongs to the fatty acid desaturase family, which is a crucial enzyme for highly unsaturated fatty acid biosynthesis. FADS3 is the third member of the fatty acid desaturase gene cluster; with at least eight evolutionarily conserved alternative transcripts (AT). FADS1 and FADS2 code for enzymes required for highly unsaturated fatty acid (HUFA) biosynthesis. FADS3 shares close sequence homology to FADS1 and FADS2 but the function of its gene product remains unknown. The three genes are clustered within 92 kb of genomic DNA located 2 kb telomeric to FEN1 and 50 kb centromeric to VMD2 and are likely to have arisen evolutionarily from gene duplication as they share a remarkably similar exon/intron organization. Protein database searches identified FADS1, FADS2, and FADS3 as fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion.

References

Zhang JY, et al. (2017) Fads3 modulates docosahexaenoic acid in liver and brain. Prostaglandins Leukot Essent Fatty Acids 123 25-32.Zhang JY, et al. (2016) Alternative splicing generates novel fads3 transcript in mice. Mol Biol Rep 43 (8): 761-766.Marquardt A, et al. (2000) Cdna cloning, genomic structure, and chromosomal localization of three members of the human fatty acid desaturase family. Genomics 66 (2): 175-183.Lin S, et al. (2018) Decidual expression and regulation of fatty acid desaturase 3 during mouse decidualization. Reproduction 156 (5): 429-437.

NCBI and Uniprot Product Information

• NCBI GI #: 74762790
• NCBI GeneID: 3995
• UniProt Accession #: Q9Y5Q0; O60426
• Molecular Weight: 445
• NCBI Official Full Name: Fatty acid desaturase 3
• NCBI Official Synonym Full Names: fatty acid desaturase 3
• NCBI Official Symbol: FADS3
• NCBI Official Synonym Symbols: CYB5RP; LLCDL3
• NCBI Protein Information: fatty acid desaturase 3; delta-9-desaturase; cytochrome b5-related protein; delta-9 fatty acid desaturase; linoleoyl-CoA desaturase (delta-9-desaturase)-like 3
• UniProt Protein Name: Fatty acid desaturase 3
• Protein Family: Fatty acid desaturase
• UniProt Gene Name: FADS3
• UniProt Synonym Gene Names: CYB5RP
• UniProt Entry Name: FADS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Uniprot Description

FADS3: is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; EC 1.14.19.-; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Biological Process: unsaturated fatty acid biosynthetic process