Rabbit anti-Human FABP4 Polyclonal Antibody | anti-FABP4 antibody

FABP4 (Fatty Acid-binding Protein, Adipocyte, Adipocyte Lipid-binding Protein, ALBP, Adipocyte-type Fatty Acid-binding Protein, A-FABP, AFABP, Fatty Acid-binding Protein 4) (FITC)

Cat. Num. AAA6377891

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FABP4; Polyclonal Antibody; FABP4 (Fatty Acid-binding Protein; Adipocyte; Adipocyte Lipid-binding Protein; ALBP; Adipocyte-type Fatty Acid-binding Protein; A-FABP; AFABP; Fatty Acid-binding Protein 4) (FITC); anti-FABP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FABP4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FABP4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length protein corresponding to aa1-132 from human FABP4.
• Immunogen Sequence: MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of FABP4 expression in human kidney using 126551.)

Western Blot (WB)

(Western Blot analysis of FABP4 expression in transfected 293T cell line by 126551. Lane 1: FABP4 transfected lysate (14.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-FABP4 antibody

Fatty acid binding proteins (FABPs) are proteins that reversibly bind fatty acids and other lipids. So far, nine tissue-specific cytoplasmic FABPs have been identified. Functions attributable to these lipid binding proteins remain unclear, but data are presented that indicate physiological roles in 1) fatty acid transport, esterification, and oxidation, 2) steroidogenesis, and 3) retinoid uptake, retinaldehyde reduction, and retinol esterification. Adipose tissue FABP (FABP4) binds both long chain fatty acids and retinoic acid. It delivers retinoic acid to nulear trans-acting proteins, thereby modulates genes coding for key proteins involved in lipid metabolism or differentiation. FABP4 is closely associated with metabolic syndrome, type 2 diabetes, and atheroscelerosis.

Product Categories/Family for anti-FABP4 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4557579
• NCBI GeneID: 2167
• NCBI Accession #: NP_001433.1
• NCBI GenBank Nucleotide #: NM_001442
• UniProt Accession #: P15090; Q6IBA1
• Molecular Weight: 132
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP
• NCBI Protein Information: fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; lipid particle; nucleus

Molecular Function: transporter activity; fatty acid binding

Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; negative regulation of protein kinase activity; cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response