ELISA (EIA)
(ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Human IgM-AP )
F(ab')2 IgM Secondary Antibody | IgM secondary antibody
Goat F(ab')2 Anti-Human IgM-AP
Gene Names
IGHM; MU; VH; AGM1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, ELISA
Synonyms
F(ab')2 IgM; Polyclonal Secondary Antibody; Goat F(ab')2 Anti-Human IgM-AP; AGM1; MU; VH; IGHM (Human); IGHM_HUMAN; IgM secondary antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
Goat F(ab')2 IgG
Specificity
Reacts with the heavy chain of human IgM
Form/Format
50mM Tris/1mM MgCl2/50% Glycerol containing <0.1% sodium azide, pH8.0
Applicable Applications for IgM secondary antibody
ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), ELISpot, Stimulation
Application Notes
Quality tested applications for relevant formats include-
ELISA 1
FLISA
Flow Cytometry 3, 4
Other referenced applications for relevant formats include-
Immunocytochemistry 15
ELISpot 2
Stimulation 5-14
ELISA 1
FLISA
Flow Cytometry 3, 4
Other referenced applications for relevant formats include-
Immunocytochemistry 15
ELISpot 2
Stimulation 5-14
Source
Pepsin digest of Goat Anti-Human IgM
Conjugation
AP (Alkaline Phosphatase)
Cross Adsorption
Human IgG and IgA; may react with IgM from other species
Preparation and Storage
Store at 2-8 degree C. Please refer to product specific SDS
ELISA (EIA)
(ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Human IgM-AP )
ELISA (EIA)
(ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Human IgM-AP )
References
1. Suryani S, Fulcher DA, Santner-Nanan B, Nanan R, Wong M, Shaw PJ, et al. Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B cells. Blood. 2010;115:519-29. (ELISA)
2. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISPOT)
3. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (FC)
4. Westera L, van Hoeven V, Drylewicz J, Spierenburg G, van Velzen JF, de Boer RJ, et al. Lymphocyte maintenance during healthy aging requires no substantial alterations in cellular turnover. Aging Cell. 2015 Jan 28. doi: 10.1111/acel.12311. [Epub ahead of print]. (FC)
5. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (Stim)
6. Gobessi S, Laurenti L, Longo PG, Sica S, Leone G, Efremov DG. ZAP-70 enhances B-cell-receptor signaling despite absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B cells. Blood. 2007;109:2032-9. (Stim)
7. Arechiga AF, Habib T, He Y, Zhang X, Zhang Z, Funk A, et al. Cutting edge: the PTPN22 allelic variant associated with autoimmunity impairs B cell signaling. J Immunol. 2009;182:3343-7. (Stim)
8. Chen K, Xu W, Wilson M, He B, Miller NW, Bengten E, et al. Immunoglobulin D enhances immune surveillance by activating antimicrobial, pro-inflammatory and B cell-stimulating programs in basophils. Nat Immunol. 2009;10:889-98. (Stim)
9. Suljagic M, Laurenti L, Tarnani M, Alam M, Malek SN, Efremov DG. Reduced expression of the tumor suppressor PHLPP1 enhances the antiapoptotic B-cell receptor signal in chronic lymphocytic leukemia B-cells. Leukemia. 2010;24:2063-71. (Stim)
10. Cheng S, Coffey G, Zhang XH, Shaknovich R, Song Z, Lu P, et al. SYK inhibition and response prediction in diffuse large B-cell lymphoma. Blood. 2011;118:6342-52. (Stim)
11. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (Stim)
12. Rankin AL, Seth N, Keegan S, Andreyeva T, Cook TA, Edmonds J, et al. Selective inhibition of BTK prevents murine lupus and antibody-mediated glomerulonephritis. J Immunol. 2013;191:4540-50. (Stim)
13. Jackson LJ, Selva S, Niedzielko T, Vollmer T. B cell receptor recognition of glatiramer acetate is required for efficacy through antigen presentation and cytokine production. J Clin Cell Immunol. 2014;5:185. (Stim)
14. Macmillan H, Strohman MJ, Ayyangar S, Jiang W, Rajasekaran N, Spura A, et al. The MHC class II cofactor HLA-DM interacts with Ig in B cells. J Immunol. 2014;193:2641-50. (Stim)
15. Lloyd KA, Wang J, Urban BC, Czajkowsky DM, Pleass RJ. Glycan-independent binding and internalization of human IgM to FCMR, its cognate cellular receptor. Sci Rep. 2017;7:42989. (ICC)
2. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (ELISPOT)
3. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (FC)
4. Westera L, van Hoeven V, Drylewicz J, Spierenburg G, van Velzen JF, de Boer RJ, et al. Lymphocyte maintenance during healthy aging requires no substantial alterations in cellular turnover. Aging Cell. 2015 Jan 28. doi: 10.1111/acel.12311. [Epub ahead of print]. (FC)
5. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (Stim)
6. Gobessi S, Laurenti L, Longo PG, Sica S, Leone G, Efremov DG. ZAP-70 enhances B-cell-receptor signaling despite absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B cells. Blood. 2007;109:2032-9. (Stim)
7. Arechiga AF, Habib T, He Y, Zhang X, Zhang Z, Funk A, et al. Cutting edge: the PTPN22 allelic variant associated with autoimmunity impairs B cell signaling. J Immunol. 2009;182:3343-7. (Stim)
8. Chen K, Xu W, Wilson M, He B, Miller NW, Bengten E, et al. Immunoglobulin D enhances immune surveillance by activating antimicrobial, pro-inflammatory and B cell-stimulating programs in basophils. Nat Immunol. 2009;10:889-98. (Stim)
9. Suljagic M, Laurenti L, Tarnani M, Alam M, Malek SN, Efremov DG. Reduced expression of the tumor suppressor PHLPP1 enhances the antiapoptotic B-cell receptor signal in chronic lymphocytic leukemia B-cells. Leukemia. 2010;24:2063-71. (Stim)
10. Cheng S, Coffey G, Zhang XH, Shaknovich R, Song Z, Lu P, et al. SYK inhibition and response prediction in diffuse large B-cell lymphoma. Blood. 2011;118:6342-52. (Stim)
11. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (Stim)
12. Rankin AL, Seth N, Keegan S, Andreyeva T, Cook TA, Edmonds J, et al. Selective inhibition of BTK prevents murine lupus and antibody-mediated glomerulonephritis. J Immunol. 2013;191:4540-50. (Stim)
13. Jackson LJ, Selva S, Niedzielko T, Vollmer T. B cell receptor recognition of glatiramer acetate is required for efficacy through antigen presentation and cytokine production. J Clin Cell Immunol. 2014;5:185. (Stim)
14. Macmillan H, Strohman MJ, Ayyangar S, Jiang W, Rajasekaran N, Spura A, et al. The MHC class II cofactor HLA-DM interacts with Ig in B cells. J Immunol. 2014;193:2641-50. (Stim)
15. Lloyd KA, Wang J, Urban BC, Czajkowsky DM, Pleass RJ. Glycan-independent binding and internalization of human IgM to FCMR, its cognate cellular receptor. Sci Rep. 2017;7:42989. (ICC)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
75,000
NCBI Official Full Name
Ig mu chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant mu
NCBI Official Symbol
IGHM
NCBI Official Synonym Symbols
MU; VH; AGM1
UniProt Protein Name
Ig mu chain C region
UniProt Gene Name
IGHM
UniProt Entry Name
IGHM_HUMAN
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Product Notes
The IgM ighm (Catalog #AAA679381) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat F(ab')2 Anti-Human IgM-AP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's F(ab')2 IgM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), ELISpot, Stimulation. Quality tested applications for relevant formats include- ELISA 1 FLISA Flow Cytometry 3, 4 Other referenced applications for relevant formats include- Immunocytochemistry 15 ELISpot 2 Stimulation 5-14. Researchers should empirically determine the suitability of the IgM ighm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F(ab')2 IgM, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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