Rabbit anti-Human FA20A Polyclonal Antibody | anti-FA20A antibody

FA20A, NT (FAM20A, Protein FAM20A) (Azide free) (HRP)

Cat. Num. AAA6297453

• Gene Names: FAM20A; AI1G; AIGFS; FP2747
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FA20A; Polyclonal Antibody; NT (FAM20A; Protein FAM20A) (Azide free) (HRP); FAM20A; Protein FAM20A; anti-FA20A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FA20A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FA20A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 48-77 in the N-terminal region of human FA20A, UniProt Accession #Q96MK3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MCF-7 cell line lysates (35ug/lane) usingMBS6003914. FA20A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human breast carcinoma tissue using MBS6003914 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of MCF-7 cells (right histogram) using MBS6003914 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FA20A antibody

Antibodies; FAM

References

Nalbant, D., et al. BMC Genomics 6 (1), 11 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 241896898
• NCBI GeneID: 54757
• NCBI Accession #: NP_060035
• NCBI GenBank Nucleotide #: NM_017565
• UniProt Accession #: Q96MK3
• Molecular Weight: 61kDa
• NCBI Official Full Name: pseudokinase FAM20A isoform a
• NCBI Official Synonym Full Names: FAM20A golgi associated secretory pathway pseudokinase
• NCBI Official Symbol: FAM20A
• NCBI Official Synonym Symbols: AI1G; AIGFS; FP2747
• NCBI Protein Information: pseudokinase FAM20A
• UniProt Protein Name: Protein FAM20A
• UniProt Gene Name: FAM20A
• UniProt Entry Name: FA20A_HUMAN

NCBI Description

This locus encodes a protein that is likely secreted and may function in hematopoiesis. A mutation at this locus has been associated with amelogenesis imperfecta and gingival hyperplasia syndrome. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Aug 2011]