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Immunofluorescence/Immunocytochemistry (IF/ICC)

Chicken F4/80 Polyclonal Antibody | anti-F4/80 antibody

Chicken Anti-F4/80 [IQF4/80 Polyclonal]

Reactivity
Mouse. Predicted to work with Rat & Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography
Synonyms
F4/80; Polyclonal Antibody; Chicken Anti-F4/80 [IQF4/80 Polyclonal]; Cell surface glycoprotein EMR1 antibody; Cell surface glycoprotein F4/80 antibody; DD7A5 7 antibody; Egf like module containing mucin like hormone receptor like 1 antibody; Egf like module containing mucin like hormone receptor like sequence 1 antibody; EGF like module receptor 1 antibody; EGF TM7 antibody; EGF-like module receptor 1 antibody; EGF-like module-containing mucin-like hormone receptor-like 1 antibody; EGFTM7 antibody; EMR 1 antibody; EMR1 antibody; EMR1 hormone receptor antibody; EMR1_HUMAN antibody; Gpf480 antibody; Ly71 antibody; Lymphocyte antigen 71 antibody; TM7LN3 antibody; anti-F4/80 antibody
Ordering
For Research Use Only!
Host
Chicken
Reactivity
Mouse. Predicted to work with Rat & Human
Clonality
Polyclonal
Isotype
IgY
Specificity
This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein which represents the glycosylated version, expressed by murine macrophages
Purity/Purification
Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography
Form/Format
Supplied in PBS containing 0.01% sodium azide and 1% trehalose
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-F4/80 antibody
ELISA (EIA), Western Blot, Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-p), Immunofluorescence (IF)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try at 1ug/ml in western blot
Immunogen
A synthetic peptide derived from C-terminus of Mouse F4/80
Target Antigen
F4/80
Preparation and Storage
Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

Immunofluorescence/Immunocytochemistry (IF/ICC)

Immunofluorescence/Immunocytochemistry (IF/ICC)

Testing Data(TD)

Testing Data(TD)

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-F4/80 antibody
Chicken Anti-F4/80 [IQF4/80 Polyclonal]

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Product Notes

The F4/80 (Catalog #AAA375282) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Chicken Anti-F4/80 [IQF4/80 Polyclonal] reacts with Mouse. Predicted to work with Rat & Human and may cross-react with other species as described in the data sheet. AAA Biotech's F4/80 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot, Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-p), Immunofluorescence (IF). Optimal antibody dilution should be determined by titration, however as a guideline try at 1ug/ml in western blot. Researchers should empirically determine the suitability of the F4/80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F4/80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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