anti-Human F11 / FXI / Factor XI Polyclonal Antibody | anti-F11 antibody

Anti-F11 / FXI / Factor XI Antibody IHC-plus

Cat. Num. AAA249882

• Gene Names: F11; FXI
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: F11 / FXI / Factor XI; Polyclonal Antibody; Anti-F11 / FXI / Factor XI Antibody IHC-plus; Sheep Polyclonal (IgG) to Human F11 / FXI / Factor XI; Human F11 / FXI / Factor XI; Coagulation factor XI; Factor xi deficiency; F11 deficiency; Factor XI; PTA deficiency; PTA; Rosenthal syndrome; FXI; anti-F11 antibody

• Host: F11/ FX1/Factor XI antibody was produced in Sheep.
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Factor XI.
• Purity/Purification: Purified
• Form/Format: ddH20, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-F11 antibody: ELISA, Western Blot
• Application Notes: IHC-Paraffin (5 ug/mL); Western Blot (10 ug/mL); ELISA (10 ug/mL); ; Optimal dilution to be determined by the researcher.
• Family/Subfamily: Protease/Serine S1
• Immunogen Species: F11/FX1/Factor XI antibody was raised against Human
• Immunogen: F11/FX1/Factor XI antibody was raised against purified human Factor XI.
• Note: During shipment,small volumes of profuct will occasionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide .The chemical,physical and toxicological properties of thsi material have not been thoroughly investigated. Standard Laboratory practices should be followed. Avoid skin and eye contact, inhalation and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent acumulations.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20° C. Aliquots are stable for at least 12 months.

Related Product Information for anti-F11 antibody

F11 Antibody, Coagulation factor XI Antibody, Factor xi deficiency Antibody, F11 deficiency Antibody, Factor XI Antibody, PTA deficiency Antibody, PTA Antibody, Rosenthal syndrome Antibody, FXI Antibody Description: F11 / FXI / Factor XI is coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds.

NCBI and Uniprot Product Information

• NCBI GI #: 4503627
• NCBI GeneID: 2160
• NCBI Accession #: NP_000119.1
• NCBI GenBank Nucleotide #: NM_000128.3
• UniProt Accession #: P03951; Q4W5C2; Q9Y495; D3DP64
• NCBI Official Full Name: coagulation factor XI preproprotein
• NCBI Official Synonym Full Names: coagulation factor XI
• NCBI Official Symbol: F11
• NCBI Official Synonym Symbols: FXI
• NCBI Protein Information: coagulation factor XI
• UniProt Protein Name: Coagulation factor XI
• Protein Family: Coagulation factor
• UniProt Gene Name: F11
• UniProt Synonym Gene Names: FXI; PTA
• UniProt Entry Name: FA11_HUMAN

NCBI Description

This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq, Jul 2008]

Uniprot Description

F11: Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. Defects in F11 are the cause of factor XI deficiency (FA11D); also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Belongs to the peptidase S1 family. Plasma kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.27; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular region; extracellular space; membrane; plasma membrane

Molecular Function: heparin binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; plasminogen activation; positive regulation of fibrinolysis

Disease: Factor Xi Deficiency