Rabbit anti-Human F10 Polyclonal Antibody | anti-F10 antibody

F10, CT (Coagulation Factor X, Stuart Factor, Stuart-Prower Factor, Factor X Light Chain, Factor X Heavy Chain, Activated Factor Xa Heavy Chain) (MaxLight 405)

Cat. Num. AAA6475507

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: F10; Polyclonal Antibody; CT (Coagulation Factor X; Stuart Factor; Stuart-Prower Factor; Factor X Light Chain; Factor X Heavy Chain; Activated Factor Xa Heavy Chain) (MaxLight 405); anti-F10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human F10.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-F10 antibody: Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), FLISA
• Application Notes: IHC-P: 1:50-1:100; FC/FACS: 1:10-1:50; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 351-381 in the C-terminal region of human F10, UniProt Accession #P00742.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of 293 cell line lysates (35ug/lane) using F0019-40. F10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(IHC analysis of in formalin fixed and paraffin embedded human hepatocarcinoma using F0019-40 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells (right histogram) using F0019-40 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-F10 antibody

Antibodies; Proteins

References

1. Alba, R., Blood 114(5), 965-971 (2009). 2. Borensztajn, K., Thromb. Res. 124(2), 219-225 (2009). 3. Larson, P.J., Biochemistry 37(14), 5029-5038 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495
• NCBI GenBank Nucleotide #: NM_000504
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X; Stuart-Prower factor; factor Xa; prothrombinase
• UniProt Protein Name: Coagulation factor X
• Protein Family: F107 fimbrial protein
• UniProt Gene Name: F10
• UniProt Entry Name: FA10_HUMAN

Uniprot Description

F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Lipid-binding; Secreted; Motility/polarity/chemotaxis; EC 3.4.21.6; Protease

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity; phospholipid binding; calcium ion binding

Biological Process: positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; blood coagulation; proteolysis; post-translational protein modification; blood coagulation, intrinsic pathway; peptidyl-glutamic acid carboxylation; positive regulation of cell migration

Disease: Factor X Deficiency