Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse F-Box And WD Repeat Domain Containing Protein 7 Polyclonal Antibody | anti-FBXW7 antibody
Polyclonal Antibody to F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)
Gene Names
Fbxw7; AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxw6; Fbxo30; SEL-10; 1110001A17Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
F-Box And WD Repeat Domain Containing Protein 7; Polyclonal Antibody; Polyclonal Antibody to F-Box And WD Repeat Domain Containing Protein 7 (FBXW7); anti-FBXW7 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FBXW7. It has beenselected for its ability to recognize FBXW7 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGNRIVSGS DDNTLKVWSA VTGKCLRTLV GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGNRIVSGS DDNTLKVWSA VTGKCLRTLV GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT
Sequence Length
176
Applicable Applications for anti-FBXW7 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
FBXW7 (Cys312~His545)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant FBXW7 (Cys312~His545) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081896
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded brain tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,547 Da
NCBI Official Full Name
F-box/WD repeat-containing protein 7 isoform 2
NCBI Official Synonym Full Names
F-box and WD-40 domain protein 7
NCBI Official Symbol
Fbxw7
NCBI Official Synonym Symbols
AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxw6; Fbxo30; SEL-10; 1110001A17Rik
NCBI Protein Information
F-box/WD repeat-containing protein 7
UniProt Protein Name
F-box/WD repeat-containing protein 7
Protein Family
UniProt Gene Name
Fbxw7
Uniprot Description
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation.
Research Articles on FBXW7
Similar Products
Product Notes
The FBXW7 fbxw7 (Catalog #AAA2028749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's F-Box And WD Repeat Domain Containing Protein 7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the FBXW7 fbxw7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CGNRIVSGS DDNTLKVWSA VTGKCLRTLV GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT. It is sometimes possible for the material contained within the vial of "F-Box And WD Repeat Domain Containing Protein 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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