Rabbit anti-Human EYA4 Polyclonal Antibody | anti-EYA4 antibody

EYA4, CT (EYA4, Eyes absent homolog 4) (FITC)

Cat. Num. AAA6297254

• Gene Names: EYA4; CMD1J; DFNA10
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EYA4; Polyclonal Antibody; CT (EYA4; Eyes absent homolog 4) (FITC); Eyes absent homolog 4; anti-EYA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EYA4 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EYA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the C-terminal region of human ADAMTS15.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EYA4 Antibody (C-term) in A2058 cell line lysates (35ug/lane). EYA4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EYA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EYA4 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EYA4 antibody

EYA4 encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti.

Product Categories/Family for anti-EYA4 antibody

Antibodies; Enzymes; Phosphatase

References

Miller, S.J. Oncogene 29 (3), 368-379 (2010). Li, H. J. Exp. Clin. Cancer Res. 28, 145 (2009). Melzer, D. PLoS Genet. 4 (5), E1000072 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 98991760
• NCBI GeneID: 2070
• NCBI Accession #: NP_004091.3
• NCBI GenBank Nucleotide #: NM_004100.4
• UniProt Accession #: O95677; O95464; O95679; Q8IW39; Q9NTR7; B7Z7F7
• Molecular Weight: 63,911 Da
• NCBI Official Full Name: eyes absent homolog 4 isoform a
• NCBI Official Synonym Full Names: EYA transcriptional coactivator and phosphatase 4
• NCBI Official Symbol: EYA4
• NCBI Official Synonym Symbols: CMD1J; DFNA10
• NCBI Protein Information: eyes absent homolog 4; dJ78N10.1 (eyes absent)
• UniProt Protein Name: Eyes absent homolog 4
• Protein Family: Eyes absent
• UniProt Gene Name: EYA4
• UniProt Entry Name: EYA4_HUMAN

NCBI Description

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

EYA4: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye. Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10). DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the HAD-like hydrolase superfamily. EYA family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein tyrosine phosphatase activity

Biological Process: anatomical structure morphogenesis; sensory perception of sound; transcription, DNA-dependent; regulation of transcription, DNA-dependent; visual perception; multicellular organismal development; chromatin modification; DNA repair

Disease: Cardiomyopathy, Dilated, 1j; Deafness, Autosomal Dominant 10