Rabbit anti-Human EXOSC8 Polyclonal Antibody | anti-EXOSC8 antibody

EXOSC8, CT (Exosome Complex Exonuclease RRP43, Ribosomal RNA-processing Protein 43, Exosome Component 8, p9, Opa-interacting Protein 2, OIP2, RRP43) (HRP)

Cat. Num. AAA6475433

• Gene Names: EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EXOSC8; Polyclonal Antibody; CT (Exosome Complex Exonuclease RRP43; Ribosomal RNA-processing Protein 43; Exosome Component 8; p9; Opa-interacting Protein 2; OIP2; RRP43) (HRP); anti-EXOSC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EXOSC8.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EXOSC8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human EXOSC8 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-EXOSC8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). EXOSC8(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with EXOSC8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EXOSC8 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EXOSC8 antibody

Antibodies; Ribosomal Proteins

References

1. Raijmakers, R., J. Mol. Biol. 323(4), 653-663 (2002). 2. Jiang, T., Proc. Natl. Acad. Sci. USA 99(8), 5295-5300 (2002). 3. Raijmakers, R., J. Mol. Biol. 315(4), 809-818 (2002). 4. Brouwer, R., J. Biol. Chem. 276 (9), 6177-6184 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 31415882
• NCBI GeneID: 11340
• NCBI Accession #: NP_852480
• NCBI GenBank Nucleotide #: NM_181503.2
• UniProt Accession #: Q96B26; O43480; Q5TBA5
• Molecular Weight: 32.4 kDa (299aa) confirmed by MALDI-TOF
• NCBI Official Full Name: exosome complex component RRP43
• NCBI Official Synonym Full Names: exosome component 8
• NCBI Official Symbol: EXOSC8
• NCBI Official Synonym Symbols: p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• NCBI Protein Information: exosome complex component RRP43
• UniProt Protein Name: Exosome complex component RRP43
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC8
• UniProt Synonym Gene Names: OIP2; RRP43; OIP-2

NCBI Description

This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2009]

Uniprot Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.