Rabbit anti-Human EXOSC1 Polyclonal Antibody | anti-EXOSC1 antibody

EXOSC1, CT (EXOSC1, CSL4, Exosome complex component CSL4, Exosome component 1) (FITC)

Cat. Num. AAA6297133

• Gene Names: EXOSC1; p13; CSL4; SKI4; Csl4p; Ski4p; hCsl4p; CGI-108
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EXOSC1; Polyclonal Antibody; CT (EXOSC1; CSL4; Exosome complex component CSL4; Exosome component 1) (FITC); Exosome component 1; anti-EXOSC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EXOSC1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EXOSC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-191 amino acids from the C-terminal region of human EXOSC1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EXOSC1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the EXOSC1 Antibody detected the EXOSC1 protein (arrow).)

Related Product Information for anti-EXOSC1 antibody

This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46.

Product Categories/Family for anti-EXOSC1 antibody

Antibodies; Proteins

References

Andersen, J.S., et al. Nature 433(7021):77-83(2005). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Deloukas, P., et al. Nature 429(6990):375-381(2004). Raijmakers, R., et al. J. Mol. Biol. 323(4):653-663(2002). Raijmakers, R., et al. J. Mol. Biol. 315(4):809-818(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 7705612
• NCBI GeneID: 51013
• NCBI Accession #: NP_057130.1
• NCBI GenBank Nucleotide #: NM_016046.3
• UniProt Accession #: Q9Y3B2; Q5JTH3; B2R9B3
• Molecular Weight: 21,452 Da
• NCBI Official Full Name: exosome complex component CSL4
• NCBI Official Synonym Full Names: exosome component 1
• NCBI Official Symbol: EXOSC1
• NCBI Official Synonym Symbols: p13; CSL4; SKI4; Csl4p; Ski4p; hCsl4p; CGI-108
• NCBI Protein Information: exosome complex component CSL4; exosomal core protein CSL4; 3'-5' exoribonuclease CSL4 homolog; CSL4 exosomal core protein homolog; homolog of yeast exosomal core protein CSL4
• UniProt Protein Name: Exosome complex component CSL4
• UniProt Gene Name: EXOSC1
• UniProt Synonym Gene Names: CSL4

Uniprot Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8.