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Western Blot (WB) (Western blot analysis of EXOC5 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). EXOC5 (arrow) was detected using the purified Pab.)

Rabbit anti-Human EXOC5 Polyclonal Antibody | anti-EXOC5 antibody

EXOC5 Antibody (C-term)

Gene Names
EXOC5; SEC10; HSEC10; SEC10P; PRO1912; SEC10L1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EXOC5; Polyclonal Antibody; EXOC5 Antibody (C-term); Exocyst complex component 5; Exocyst complex component Sec10; hSec10; SEC10; SEC10L1; anti-EXOC5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EXOC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-66 amino acids from the C-terminal region of human EXOC5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
40-66
Sequence Length
708
Applicable Applications for anti-EXOC5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of EXOC5 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). EXOC5 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of EXOC5 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). EXOC5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EXOC5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EXOC5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EXOC5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (EXOC5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-EXOC5 antibody
EXOC5 is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.
Product Categories/Family for anti-EXOC5 antibody
References
Kee,Y., et.al., Proc. Natl. Acad. Sci. U.S.A. 94 (26), 14438-14443 (1997)
Hsu,S.C., et.al., Neuron 20 (6), 1111-1122 (1998)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81853
NCBI Official Full Name
exocyst complex component 5
NCBI Official Synonym Full Names
exocyst complex component 5
NCBI Official Symbol
EXOC5
NCBI Official Synonym Symbols
SEC10; HSEC10; SEC10P; PRO1912; SEC10L1
NCBI Protein Information
exocyst complex component 5
UniProt Protein Name
Exocyst complex component 5
Protein Family
UniProt Gene Name
EXOC5
UniProt Synonym Gene Names
SEC10; SEC10L1; hSec10
UniProt Entry Name
EXOC5_HUMAN

NCBI Description

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. [provided by RefSeq, Jul 2008]

Uniprot Description

EXOC5: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Belongs to the SEC10 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: Ral GTPase binding; protein N-terminus binding

Biological Process: protein transport; cellular protein metabolic process; exocytosis; vesicle docking; organelle organization and biogenesis; post-Golgi vesicle-mediated transport

Research Articles on EXOC5

Similar Products

Product Notes

The EXOC5 exoc5 (Catalog #AAA9213714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 40-66. The EXOC5 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EXOC5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the EXOC5 exoc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EXOC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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