Western Blot (WB)
(Western blot analysis of EXO1 expression in Raw264.7 (A), A431 (B) whole cell lysates.)
Rabbit anti-Human, Mouse EXO1 Polyclonal Antibody | anti-EXO1 antibody
Anti-EXO1 Antibody
Western Blot (WB)
(Western blot analysis of EXO1 expression in Raw264.7 (A), A431 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EXO1 expression in Raw264.7 (A), A431 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of EXO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of EXO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: nucleoplasm; nucleus
Molecular Function: 5'-3' exonuclease activity; protein binding; structure-specific DNA binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; DNA binding; 5'-3' exodeoxyribonuclease activity; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity; metal ion binding; exonuclease activity; ribonuclease H activity; flap endonuclease activity
Biological Process: humoral immune response mediated by circulating immunoglobulin; mismatch repair; meiotic cell cycle; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination
Research Articles on EXO1
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Product Notes
The EXO1 exo1 (Catalog #AAA821599) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EXO1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EXO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC), Chromatin Immunoprecipitation (ChIP). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500), ChIP (1/100 - 1/500). Researchers should empirically determine the suitability of the EXO1 exo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EXO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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