Rabbit EWSR1 Polyclonal Antibody | anti-EWSR1 antibody

EWSR1 antibody - middle region

Cat. Num. AAA3205448

• Gene Names: EWSR1; EWS; EWS-FLI1; bK984G1.4
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: EWSR1; Polyclonal Antibody; EWSR1 antibody - middle region; anti-EWSR1 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: QPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQS
• Sequence Length: 656

• Applicable Applications for anti-EWSR1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EWSR1
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-EWSR1 antibodyParaffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

Western Blot (WB)

(Lanes:1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Goat anti-Rabbit HRPSecondary Antibody Dilution:1:4000Gene Name:EWSR1Submitted by:Dr. Crissy Dudgeon, Ph.D., CINJ)

Western Blot (WB)

(Lanes:Lane 1: 50ug Hela LysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:10,000Gene Name:EWSR1Submitted by:Archa Fox, University of Western AustraliaEWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

Western Blot (WB)

(Sample Type: HEK cells transfected with pDYKDDDDK EWSR1-ATF1Primary Dilution: 1:1000EWSR1 is supported by BioGPS gene expression data to be expressed in HEK293)

Western Blot (WB)

(WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

Related Product Information for anti-EWSR1 antibody

This is a rabbit polyclonal antibody against EWSR1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: EWSR1 is a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.

Product Categories/Family for anti-EWSR1 antibody

Polyclonal; RNA Binding Proteins; Transcription Regulation; Disease Related; DNA/RNA/Protein Interactions; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 253970502
• NCBI GeneID: 2130
• NCBI Accession #: NP_005234
• NCBI GenBank Nucleotide #: NM_005243
• UniProt Accession #: Q01844
• Molecular Weight: 68kDa
• NCBI Official Full Name: RNA-binding protein EWS isoform 3
• NCBI Official Synonym Full Names: EWS RNA binding protein 1
• NCBI Official Symbol: EWSR1
• NCBI Official Synonym Symbols: EWS; EWS-FLI1; bK984G1.4
• NCBI Protein Information: RNA-binding protein EWS
• UniProt Protein Name: RNA-binding protein EWS
• Protein Family: RNA-binding protein
• UniProt Gene Name: EWSR1
• UniProt Synonym Gene Names: EWS
• UniProt Entry Name: EWS_HUMAN

NCBI Description

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]

Uniprot Description

EWS: an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described.

Protein type: Oncoprotein; Transcription, coactivator/corepressor; RNA-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: calmodulin binding; identical protein binding; protein binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Ewing Sarcoma