Rabbit anti-Human ETS2 Polyclonal Antibody | anti-ETS2 antibody

ETS2, ID (ETS2, Protein C-ets-2) (MaxLight 490)

Cat. Num. AAA6296949

• Gene Names: ETS2; ETS2IT1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ETS2; Polyclonal Antibody; ID (ETS2; Protein C-ets-2) (MaxLight 490); Protein C-ets-2; anti-ETS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ETS2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ETS2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-173 in the central region of human ETS2, UniProt Accession #P15036; P15037; A1A4L6. Species sequence homology: bovine, mouse
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS6012380. ETS2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue using MBS6012380 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6012380 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MCF-7 cells using MBS6012380 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ETS2 antibody

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.

Product Categories/Family for anti-ETS2 antibody

Antibodies; Transcription Factors

References

1. Zabuawala, T., et al. Cancer Res. 70(4):1323-1333(2010). 2. Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). 3. Shalaby, A.A., et al. Mod. Pathol. 22(8):996-1005(2009). 4. Sutter, W., et al. J Craniomaxillofac Surg 37(5):263-271(2009). 5. Staun-Ram, E., et al. Reprod. Biol. Endocrinol. 7, 135 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119645
• NCBI GeneID: 2114
• UniProt Accession #: P15036; A1A4L6
• Molecular Weight: 469
• NCBI Official Full Name: Protein C-ets-2
• NCBI Official Synonym Full Names: v-ets avian erythroblastosis virus E26 oncogene homolog 2
• NCBI Official Symbol: ETS2
• NCBI Official Synonym Symbols: ETS2IT1
• NCBI Protein Information: protein C-ets-2; oncogene ETS-2; v-ets erythroblastosis virus E26 oncogene homolog 2; v-ets avian erythroblastosis virus E2 oncogene homolog 2
• UniProt Protein Name: Protein C-ets-2
• Protein Family: Protein
• UniProt Gene Name: ETS2
• UniProt Entry Name: ETS2_HUMAN

NCBI Description

This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Ets-2: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus E26 oncogene. A target of the Akt/JNK signaling pathway in macrophages. A PKC pathway activates ETS2, up-regulating GM-CSF in non-small lung carcinoma cells.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; skeletal development