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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Rabbit anti-Human, Mouse Ets1 Polyclonal Antibody | anti-Ets1 antibody

Phospho-Ets1 (Thr38) Antibody

Gene Names
Ets1; Tpl1; Ets-1; AI196000; AI448617; D230050P06
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Synonyms
Ets1; Polyclonal Antibody; Phospho-Ets1 (Thr38) Antibody; p54; ETS1; ETS-1; C-ets-1 protein; anti-Ets1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Form/Format
Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Applicable Applications for anti-Ets1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:200
Immunogen
Peptide sequence around phosphorylation site of threonine 38(L-L-T(p)-P-S) derived from Human ETS1.
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody.)
Product Categories/Family for anti-Ets1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
50,202 Da
NCBI Official Full Name
c-ets-1
NCBI Official Synonym Full Names
E26 avian leukemia oncogene 1, 5' domain
NCBI Official Symbol
Ets1
NCBI Official Synonym Symbols
Tpl1; Ets-1; AI196000; AI448617; D230050P06
NCBI Protein Information
protein C-ets-1; avian erythroblastosis virus E26 oncogene 1; p54; tumor progression locus 1
UniProt Protein Name
Protein C-ets-1
Protein Family
UniProt Gene Name
Ets1
UniProt Entry Name
ETS1_MOUSE

Uniprot Description

Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; immune system process; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; positive regulation of cell motility; regulation of angiogenesis; negative regulation of cell cycle; response to antibiotic; regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; positive regulation of angiogenesis; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; positive regulation of cell migration

Research Articles on Ets1

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Product Notes

The Ets1 ets1 (Catalog #AAA7133542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Ets1 (Thr38) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ets1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:200. Researchers should empirically determine the suitability of the Ets1 ets1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ets1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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