Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ETFB/FP585 Polyclonal Antibody | anti-ETFB antibody
Goat anti-ETFB/FP585 (aa 152-165) Antibody
Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 27kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ETFB: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B). GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF beta-subunit/FixA family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion
Molecular Function: electron carrier activity
Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase
Disease: Multiple Acyl-coa Dehydrogenase Deficiency
Research Articles on ETFB
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Product Notes
The ETFB etfb (Catalog #AAA422410) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ETFB/FP585 (aa 152-165) Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ETFB/FP585 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml. Western Blot: Approx 27kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ETFB etfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QVTLEGDKLK VERE. It is sometimes possible for the material contained within the vial of "ETFB/FP585, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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