Rabbit EST1A Polyclonal Antibody | anti-SMG6 antibody

EST1A Antibody

Cat. Num. AAA9612131

• Gene Names: SMG6; EST1A; SMG-6; C17orf31; hSMG5/7a
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: EST1A; Polyclonal Antibody; EST1A Antibody; C17orf31; EST1-like protein A; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; Smg-6 homolog; SMG6; Telomerase subunit EST1A; Telomerase-binding protein EST1A; anti-SMG6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EST1A Antibody detects endogenous levels of total EST1A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 511

• Applicable Applications for anti-SMG6 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human EST1A, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, using EST1A Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612131 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612131) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-SMG6 antibody

Function: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PubMed:19179534). May have a general role in telomere regulation (PubMed:12676087, PubMed:12699629). Promotes in vitro the ability of TERT to elongate telomeres (PubMed:12676087, PubMed:12699629). Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization (PubMed:12676087, PubMed:12699629). Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER) (PubMed:12676087, PubMed:12699629).Plays a role in nonsense-mediated mRNA decay (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788). Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788). Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788).

Subcellular Location: Nucleus>Nucleolus. Chromosome>Telomere. Cytoplasm>Cytosol. Note: Particularly enriched in the nucleolus.

Tissue Specificity: Ubiquitous.

Subunit Structure: May form homooligomers (PubMed:20930030). Associated component of the telomerase holoenzyme complex (PubMed:19179534). Interacts with TERT, independently of the telomerase RNA (PubMed:12676087, PubMed:12699629). Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits (PubMed:12554878, PubMed:19060897, PubMed:20930030). Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay (PubMed:20930030).

Similarity: The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.

NCBI and Uniprot Product Information

• NCBI GI #: 378744173
• NCBI GeneID: 23293
• NCBI Accession #: NP_001243756.1
• NCBI GenBank Nucleotide #: NP_001243756.1
• UniProt Accession #: Q86US8
• Molecular Weight: Observed Molecular Weight: (Observed)160kD.; Predicted Molecular Weight: (Calculated)160kDa.
• NCBI Official Full Name: telomerase-binding protein EST1A isoform 3
• NCBI Official Synonym Full Names: SMG6 nonsense mediated mRNA decay factor
• NCBI Official Symbol: SMG6
• NCBI Official Synonym Symbols: EST1A; SMG-6; C17orf31; hSMG5/7a
• NCBI Protein Information: telomerase-binding protein EST1A
• UniProt Protein Name: Telomerase-binding protein EST1A
• Protein Family: Telomerase-binding protein
• UniProt Gene Name: SMG6
• UniProt Synonym Gene Names: C17orf31; EST1A; KIAA0732
• UniProt Entry Name: EST1A_HUMAN

NCBI Description

This gene encodes a component of the telomerase ribonucleoprotein complex responsible for the replication and maintenance of chromosome ends. The encoded protein also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD. Alternatively spliced transcript variants encoding distinct protein isoforms have been described. [provided by RefSeq, Feb 2014]